Wednesday December 11th, 2019
Lecture by Carlas Smith (Delft University of Technology, The Netherlands)
Where: UAntwerp, Campus Drie Eiken, building N, room N.1.08
Universiteitsplein 1, 2610 Antwerpen
Time: 15:00 AM - 15:45 PM
Uniting Structured Illumination and Localization Microscopy (SIMFLUX)
MINFLUX offers a breakthrough in single molecule localization precision, but suffers from a tiny field of-view and a lack of practical parallelism. SIMFLUX makes use of extended illumination patterns similar to those used in Structured Illumination Microscopy (SIM). Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron sized field-of-view. We show a near twofold improvement in precision over standard localization microscopy with the same photon count on DNA-origami nano-structures and Tubulin, imaged with PAINT and dSTORM.
 Jelmer Cnossen, Taylor Hinsdale, Ramsus Thorsen, Florian Schueder, Ralf Jungmann, Carlas S Smith, Bernd Rieger, Sjoerd Stallinga, Localization microscopy at doubled precision with patterned illumination, bioRxiv 554337;