Available equipment

Can be used for…

LaVision Ultraview II Light Sheet Microscope

Imaging physiologically relevant specimens such as organoids, whole organs and intact small organisms fast and with minimal photodamage. Combined with in-house optimized chemical tissue clearing strategies, light sheet-based microscopy allows for non-destructive, high-resolution, volumetric imaging of the intact mouse brain. It is amply used for imaging pathological hallmarks such as amyloid and tau aggregates, as well as vasculature and glial cell distribution in mouse brain. This system has also been used for gut, kidney, liver and whole lung.

Confocal Laser Scanning 

Platform Leica TCS SP8 with

light sheet module

This system allows visualizing cells, tissue specimen and living small model organisms in three dimensions with excellent resolution and contrast. In addition to the high-resolution multispectral imaging that this system can offer, the platform is equipped with a light-sheet module for live imaging of small organisms (such as zebrafish) and small organoids (e.g. tumor spheroids or neurospheres). A white light laser offers full spectral flexibility at the excitation side, while spectral detectors enable deconvolution of overlapping fluorochromes. The device has a broad application radius in the neurosciences domain for studying neurodegeneration and -regeneration (e.g. whole brain imaging, optogenetics); but it also has direct utility in various other fields such as cardiovascular research (e.g. plaque formation and stability), plant developmental research (e.g. protein localization during plant growth) and ecotoxicology (e.g. teratogenicity and developmental defects in zebrafish.

PerkinElmer UltraVIEW Vox spinning disk confocal system

PerkinElmer UltraVIEW ERS spinning disk confocal system

Spinning disk microscopy allows studying the dynamics of subcellular processes and molecular fluxes in cells with high spatiotemporal resolution. One system (ERS) is geared towards in fluxoanalysis for online compound stimulation or medium exchange, whereas the other (VOX) is fully automated and is typically used for long-term minimally invasive multi-well plate acquisitions or calcium imaging.

Nikon Ti2 W1 spinning disk with SoRa, photoablation and selective illumination

This multimodal system is equipped for ultrafast live cell imaging (400fps in widefield mode and 200fps confocal mode), ratiometric imaging for concentration-independent flux analysis (e.g. FURA2), precise photoablation, and selective multi-region photoconversion (e.g. for optogenetics). It’s SoRA module and associated deconvolution software allow for superresolution imaging and built-in AI and JOBS modalities enable online image recognition and adaptation of imaging modalities. 

Nikon Ti with automatic stage loader

This fully automated system is dedicated to cell-based screens as well as slide scanning (virtual pathology). The associated stage loader has a capacity for automatic feeding of 20 plates and 80 slides. It is used for capturing large image data sets (i.e. high-content imaging) and is combined with in-house available expertise in high-content image analysis (i.e. exhaustive feature extraction, yielding high information content). This platform is an interesting screening tool in pre-clinical research, e.g. for neurotoxicity screening over target identification/validation to phenotypic drug screening on 

in vitro disease models.

Zeiss Axioscan

Digital pathology involves high-speed, high-resolution digital acquisition of images representing entire stained tissue sections from glass slides and allows them to be viewed directly in much the same way as standard microscopy. While this creates a permanent record of histological slide data and facilitates data sharing with collaborators, importantly, it allows analysis, quantification and objective pathological assessment of entire tissue samples, which is now current practice in pre-clinical and clinical research. The system has the capacity to digitize up to 100 slides in one run. 

Leica Laser Microdissection LMD7000 

Laser microdissection technology enables molecular interrogation of specific, selected regions within a tissue section. Defined regions such as tumours or even single cells can be cut out of tissue sections and captured into a separate container for downstream analyses (e.g. qPCR or sequencing). 

Tecnai Transmission Electron Microscope with cryo-tomography

Transmission electron microscopy (TEM) allows investigating the ultrastructure of biological samples, i.e., tissues and cells, at the nanometre scale (down to 0.1 nm). It is being used for visualizing  autophagy, mitochondrial or ER stress or viral and bacterial infections. TEM is currently also an important tool for the identification of extravesicular vesicles, and for visualizing cell-nanoparticle (e.g. gold) interactions. Immunogold labelling offers a means to identify specific proteins and the cryo-tomography module enables volumetric imaging of macromolecular complexes.

Scanning Electron Microscope Jeol JSM-T100

Scanning electron microscopy is primarily used for visualising the surface (i.e. topology) of tissues and cells. SEM has a broad range of applications, including investigating the gut epithelium, cilia of lung tissue and is amply used for established assays such as the Drosophila rough eye screen.