Nikon Ti2 W1 spinning disk with SoRa, photoablation and selective illumination

SoRa
Description:
Nikon Ti2 W1 spinning disk with SoRa, photoablation and selective illumination
Applications:
Multimodal system equipped for fast live cell imaging, ratiometric imaging for concentration-independent flux analysis (e.g. FURA2), precise photoablation, and selective multi-region photoconversion (e.g. for optogenetics). SoRA module and associated deconvolution software allow for superresolution imaging and built-in AI and JOBS modalities enable online image recognition and adaptation of imaging modalities.
Samples:
Biological and clinical samples, i.e. cell cultures, tissue sections, vibratome slices (fixed and living samples)
Light Source:
LaserCombiner with 4 lasers and 2 fibers (405, 488, 561, 640 nm)
355 nm pulsed laser for photo-ablation

Specifications:
​- W1-SoRa spinning disk with 50 µm SuperResolution and 50 µm regular confocal spinning disk
- 2 camera's: 
 Photometrics Kinetix sCMOS (6,5 µm pixel)
 Photometrics Prime95B sCMOS camera (11 μm pixel)
- Perfect Focus System
- Photomanipulation device UV+VIS OMS including 355 nm pulsed laser for photo-ablation and input for visible laser.
- Photomanipulation device DMD with distinct illumintor CoolLED PE800
- Ratiometric illumination device Fura2+
- JOBS Smart Imaging Design- Artificial Intelligence analysis modules
- Incubation chamber for temperature and CO2,

Funding:
The Nikon Ti2 W1 spinning disk was purchased with support of the Hercules Foundation.

Confocal Laser Scanning Platform Leica TCS SP8 light sheet microscopy

Leica_1000.jpg
Description:
Inverted Leica TCS SP8 confocal laser scanning microscope with light sheet module
Applications:
Optical sectioning, 3D-imaging, Live Cell Imaging, automated recordings

FRAP, FRET

Light sheet: deep tissue imaging of thick, transparent specimen
Samples:
Biological and clinical samples, i.e. cell cultures, tissue sections, vibratome slices and cleared specimens for light sheet
Light Source:
White Light laser, 405 laser line, Argon laser
Specifications:
White light laser for excitation of any dye covering the full visible spectrum, use of 8 excitation lines simultaneously

2 HyD detectors for multi-spectral imaging and 2 PMT detectors

DLS digital light sheet module

Environmental chamber with CO2 and temperature  control
Funding:
The Leica TCS SP8 confocal system with light sheet was purchased with support of the Hercules Foundation.

PerkinElmer UltraVIEW Vox spinning disk confocal system

Description: Inverted Nikon Ti wide-field microscope equiped with a micro-lenses enhanced spinning disk confocal system and photokinesis unit
Applications: High speed/ high resolution imaging of (living) specimens with minimal photobleaching

Optical sectioning, 3D-imaging, live cell imaging (Ca2+ imaging, cell trafficking,..)

FRAP, FRET
Samples: Biological and clinical samples, i.e. cell cultures, tissue sections, vibratome slices,plants …
Light Source: Six solid-state lasers: 405, 440, 488, 514, 561, 640nm
Specifications: EM-CCD detector for high speed imaging

Photokinesis unit for FRET and FRAP applications

Environmental chamber with CO2 en temperature  control

Volocity software for deconvolution, colocalisation, cell trafficking, 3D-imaging,…
Funding: The PerkinElmer UltraVIEW Vox was purchased with support of the Hercules Foundation

PerkinElmer UltraVIEW ERS spinning disk confocal system

Description: Inverted Zeiss wide-field microscope equiped with a micro-lenses enhanced spinning disk confocal system
Applications: High speed/ high resolution imaging of (living) specimens with minimal photobleaching Optical sectioning, 3D-imaging, live cell imaging (Ca2+ imaging, cell trafficking,..)
Samples: Biological and clinical samples, i.e. cell cultures, tissue sections, vibratome slices,plants …
Light Source: Argon-Krypton laser: 488, 568, 647nm
Specifications: CCD camera for high speed imaging

Inverted microscope with on stage CO2 -incubator and temperature control

Volocity software for deconvolution, colocalisation, cell trafficking, 3D-imaging,…

Zeiss LSM510 META

Description: Inverted Zeiss wide-field microscope with a confocal laser scanning system for high resolution imaging of multiple fluorescent labels
Applications: High resolution imaging of multiple fluorescent labels Optical sectioning, 3D-imaging
Samples: Biological samples (fixed/non-fixed): cells, tissues, plants,…
Light Source: Argon and Helium Neon gas lasers: 458, 477, 488, 518, 543, 633nm
Specifications: Inverted microscope

2 PMT detectors

Meta Spectral detector allowing multichannel unmixing