Whole Genome Sequencing

With a variety of sequencing instruments available at the LMM, we have the expertise in providing services in whole genome sequencing. We have developed a whole genome sequencing analysis pipeline for bacterial genomes named BacPipe which is pending publication and will be made open-access when published.

BacPipe is capable of performing de novo and hybrid genome assemblies combining long-read sequencing with short-read Illumina data to close the gaps in the genome assemblies generated by short-read sequences and identify mis-assemblies.

MinION and PacBio Sequel can be utilised to obtain complete chromosomes, including plasmids, plastids, and mitochondrial sequences. PacBio can also be used to determine epigenetic modifications (methylation), targeted sequencing, and detect isoforms.

The LMM has the capability of conducting metagenomics analysis for microbial composition and taxonomical diversity by 16SrDNA, Internal Transcribe Spacer (ITS), and by shotgun sequencing.