Experimental models

Animal models:

·         MNU-Helicobacter pylori mouse model for gastric cancer (C57BL/6; wildtype & Muc13 KO, Muc1 KO mice)

·         DSS colitis model (C57BL/6; wildtype & Muc13 KO, Muc1 KO mice)

·         TNBS colitis model (mouse; rat)

·         Sepsis: postoperative ileus and septic ileus (cecal ligation and puncture; LPS; mouse)

·         Portal hypertension: common bile duct ligation (rat)

·         NAFLD/NASH: MCD diet; HFD (+/- fructose; rat – mouse)

·         Hepatocellular autophagy-deficient mouse model

Organoids models derived from inflammatory bowel disease (IBD) patients


Molecular biological techniques (RT-qPCR, Western blotting, ELISA, immunohistochemistry)

Genotyping (PCR and agarose electrophoresis)

In vitro gastrointestinal contractility (organ bath) In vitro gastrointestinal peristalsis (adapted Trendelenburg assay) In vivo gastrointestinal motility (gastric emptying, GI transit, Evans Blue, beads) Visceromotor response (VMR) to colorectal distension In vitro afferent nerve recording In vivo portal blood flow

In vitro intrahepatic vascular resistance (liver perfusion, organ bath)

Next-generation sequencing technologies:

-          Targeted isoform sequencing (PacBio, Nanopore)

-          16s rRNA gene sequencing (Illumina)

-          Shotgun sequencing (metagenomics; Illumina)