Addressing the specific research interests of the laboratory requires a multidisciplinary approach. Part of the research team´s expertise and equipment is listed below.
equipment for electrophysiology
Four high-resolution patch-clamp setups for analyzing primary cell cultures or transiently/stably transfected cell lines, 2 of the setups are equipment with a pressurized fast-switching perfusion system for administering drugs/toxins.
One cut-open vaseline gap oocyte setup able to perform state-of-the-art voltage-clamp fluorometry (VCF) experiments.
molecular biology and biochemistry techniques
Laboratory has a large collection of ion channel clones (mainly Kv channels) and the standard molecular biology equipment for cloning new channels or creating channel mutations.
cell culture and expression systems
L2 cell culture facility equipped with two laminar flow hoods and 4 CO2 incubators. Common cell lines for heterologous expression of proteins (e.g. HEK293, CHO, Ltk-,…) are part of our routine cell culture repertoire. Cells can be transiently or stably transfected according to the experimental needs.
Facility to express channel proteins in Xenopus laevis oocytes (e.g. for performing VCF experiments).