Field techniques used: Bird trapping (mist nets, funnel traps, nest-boxes), biometric measurements, bird ringing, blood / fat / preen oil sampling (non-destructive), feces / pellets collection.
Laboratory techniques used: Chromatography (HPLC-MS, GC-MS) , spectroscopy (AA), ELISA, genetics (PCR), genomics and transcriptomics (RT-PCR).
These techniques are used to perform the following analyses:
Toxicological analyses: Pesticides, PFAAs, rodenticides, metals.
Antioxidants and oxidative stress parameters: Total non-enzymatic antioxidant capacity (TAC), glutathione peroxidase (GPX) / superoxide dismutase (SOD) / catalase (CAT) activity, protein carbonyls, lipid peroxidation (MDA), reduced (GSH) and oxidized glutathione (GSSG), vitamins (retinol, α-tocopherol) and carotenoids (zeaxanthin and lutein).
Immune parameters: Innate immune parameters (agglutination, lysis, haptoglobin concentrations and nitric oxide concentrations), cellular immune response (phytohemagglutinin (PHA) skin test), humoral immune response (haemagglutination test after injecting an antigen, SRBC).
Plasmatic biochemistry: alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LHD), creatine phosphokinase (CPK), albumin, total protein, glucose, cholesterol, triglycerides, calcium, magnesium, phosphorus, creatinine, urea and uric acid.
Hormone concentration: Sex steroid hormones (testosterone and estradiol) and thyroid hormones (thyroxine (T4), triiodothyronine (T3)).
Transcriptomics: RNA extraction, RT-PCR, high throughput sequencing.
Other measurements: Molecular sexing of birds, acetylcholinesterase activity (AChE).
Industry (Chemical industry to perform studies about the effects of pollutants in birds).
Wildlife, Toxicology, Conservation, Risk assessment