ALP isoenzyme patterns are obtained by means of agarose electrophoresis.
Identification of the fractions is based on their electrophoretic migration pattern, as well as on changes of this pattern following specific sample treatment, such as heat treatments, incubation with neuraminidase, anti-placental / anti-intestinal ALP antibodies etc.
The clinical interpretation of the ALP isoenzyme patterns is performed with help of a rule-based expert system that we developed in ProMD, a medical expert system shell.
Potential users are clinicians and researchers.
Separating alkaline phosphatase into its isoforms/isoenzymes is particularly valuable in patients with malignant disease (bone and liver metastasis), in patients with bone diseases (e.g. Paget's disease of bone, vitamin D deficiency, osteomalacia, osteoporosis, hyper/hypoparathyroidism ..), in patients with liver diseases (hepatitis, cholestatic disease ..), in children (growth deficiency, transient hyperphosphatasemia, rickets, liver disease ..) and in patients with chronic renal insufficiency (adynamic bone disease, uremic osteodystrophy ..).
Paget disease, Bone matrix proteins, Mineralisation, Vitamin d, Matrix components, Isoenzymes, Alkaline phosphatase, Expert systems