Transmission Electron Microscopy (TEM)
Compared to optical microscopy, the TEM technique has much higher resolution making it the technique for visualization of subcellular structures (like mitochondria, vacuoles, viruses, etc.).
A cryo transmission electron microscope (Tecnai G2 Spirit BioTWIN) is available to study individual molecules or macromolecular assemblies with a minimum of sample preparation artifacts. This high-contrast, high-resolution microscope operates up to 120 kV and achieves magnifications ranging from 2200x to 300,000x, yielding a 0.34 nm line resolution. Furthermore, the lab has expertise for cryo sample preparation and TEM-tomography to study naive conformations of proteins and intracellular structures.
TEM is used within EGAMI and its partner network for diagnostics (collaborations with the clinical pathology department), study of autophagy (and other types of cell death), microbiology, infectious diseases and parasitology etc.
Scanning electron microscopy (SEM)
To complete our electron microscopic facility we have a Phillips SEM 515 (30 kV, 160,000x magnification) scanning electron microscope, perfectly capable of visualizing surfaces for biological and material sciences.