Subfertility is a global public health issue. The main causes of subfertility are metabolic diseases such as obesity or type 2 diabetes. Also in farm animals, the link between metabolic stress and subfertility has been acknowledged. Maternal metabolic disorders are associated with upregulated lipolysis leading to a disturbed metabolic profile in the blood (increased non-esterified fatty acids (NEFAs), ketones, altered glucose and insulin). Using the cow as a model, we showed that this altered metabolic profile is reflected in the follicular and oviductal environment in which the oocyte and embryo develop, subsequently altering oviductal functions and jeopardizing oocyte and embryo development and quality. More mechanistic research revealed that increased oxidative stress is one of the key underlying pathways associated with metabolic stress at oocyte and embryo level. Therefore, antioxidants may play an important role in alleviating oxidative stress in metabolically compromised embryos, hereby ameliorating embryo developmental competence and quality and thus fertility. We already showed that circulating antioxidants are reflected in the oocyte's micro-environment and can be altered by the diet. Little is known about the antioxidative environment of the pre-implantation embryo (in the oviduct) and whether these antioxidants can influence the development and quality of metabolically stressed embryos. As such, we hypothesize that dietary antioxidant supplementation in metabolically compromised mothers can change oviductal concentrations of antioxidants hereby changing oviductal cell functions and ameliorating embryo developmental competence and quality. Therefore, the objective of the research proposed here is to culture in vitro derived bovine zygotes in the oviducts of metabolically stressed ewes supplemented with antioxidants and investigate the antioxidative status of the oviductal fluid, the characteristics of the oviduct at the transcriptomic level and the embryo developmental competence and quality. Enhancing the oviductal environment to better support early embryo development is crucial for the embryos to establish successful pregnancy later in development. These results can contribute to drastically reduced medical costs for subfertility treatments. In addition, they may tackle the subfertility problem in high yielding dairy cows hereby improving sustainable and profitable farming.