Although hypoxia-related disorders (stroke, heart attack, ') count for a substantial amount of the mortality rate in western society, the underlying pathophysiology is still obscure. Until now there are only a few hypoxia-inducible proteins characterized that help to counteract the adverse effects of hypoxic/ ischemic injury and determine the fate of cells.
Identifying proteins that are differentially expressed in hypoxic and non-hypoxic brain could help illuminate pathophysiology and provide novel therapeutic targets in cerebral ischemia.
To that end the response of in vitro and in vivo neuronal models to oxygen depletion will be studied at protein and RNA level, by use of two-dimensional electrophoresis and microarray analysis. The results of both techniques will be compared in order to get a complete and correct list of proteins/ genes that are involved in the cellular adaptation to cerebral hypoxia.