Real-time reconstruction of arbitrary slices for quantitative and in-situ electron tomography

Datum: 14 februari 2020

Locatie: UAntwerp, Campus Groenenborger, Room U.408 - Groenenborgerlaan 171 - 2020 Antwerp (Wilrijk) (route: UAntwerpen, Campus Groenenborger)

Tijdstip: 11.30 - 12.15 uur

Organisatie / co-organisatie: EMAT

Korte beschrijving: Friday Lecture by Hans Vanrompay, EMAT

About the lecture

Electron tomography experiments are typically composed out of several consecutive steps; acquisition, reconstruction, and analysis/quantification. Usually, the latter two steps are performed offline using a dedicated workstation. This sequential workflow unfortunately prevents on-the-fly control of experimental parameters to improve the quality of the 3D reconstruction or steer the tomographic experiment. Here, we present an efficient approach to address this key constraint, based on the real-time reconstruction of arbitrary 2D reconstructed slices through a 3D object. We demonstrate that this approach is essential for the real-time assessment of both the structure under investigation as well as the used experimental parameters, hence for improving the efficiency of the electron tomography experiment. Furthermore, we show that for an in-situ investigation, such real-time qualitative and quantitative information is indispensable for adaptively steering the in-situ parameters during a tomography experiment.



Figure: Illustration of the workflow of a conventional tomography experiment (dashed arrows). First, the projection images are acquired. The direction of the electron beam is indicated by the red arrow. Next, a complete 3D reconstruction is performed. Finally, orthogonal slices through the 3D reconstruction are investigated through 2D orthoslices. RECAST3D provides a new approach (full line) in which user selected oblique slices are reconstructed on demand.