Basal activity and regulation of endothelial nitric oxide synthase in the mouse aorta
17 april 2014
UAntwerp - Campus Drie Eiken - Building Q - Promotiezaal - Universiteitsplein 1 - 2610 Wilrijk
Johanna van Langen
prof. dr. Hidde Bult
PhD defense Johanna van Langen - Faculty of Medicine and Health Sciences
Nitric oxide (NO) regulates vascular tone and maintains the vessel wall in an anti-inflammatory state, thereby protecting against atherosclerosis. Although basal NO production by endothelial nitric oxide synthase (eNOS) is responsible for the maintenance of vascular compliance and homeostasis, most research in patients and animal models of vascular disease focuses on agonist-induced NO production, whereas basal eNOS activity is rarely investigated. This thesis aimed to elucidate changes in basal NO production in vascular disease and to unravel the discrepancies between basal and agonist-stimulated eNOS activity. Basal NO production was found attenuated in several mouse models for vascular disease. Moreover, numerous evidences were found for the discrepancy between basal and agonist-stimulated eNOS activity and part of the signalling pathway responsible for basal eNOS activity was revealed.