Integrated Molecular Practical

Course Code :1022FBDBIC
Study domain:Biochemistry
Academic year:2019-2020
Semester:2nd semester
Sequentiality:min. 08/20 for Laboratory exercises. Cannot be followed with courses from 1st bachelor. Simultaneous registration or min 08/20 for Mol. gen. I and Mol. gen. II and Mol. en cell. immunology and Bio-informatics
Contact hours:120
Study load (hours):168
Contract restrictions: Exam contract not possible
Language of instruction:Dutch
Exam period:exam in the 2nd semester
Lecturer(s)Eva Geuens
Ingrid De Meester
Julie van der Zee

3. Course contents *

The practical exercises consist out of three parts.

1) Molecular and cellular immunology: students learn techniques base don antigen-antibidy interactions as ELISA, immuno-precipitaion, IgG-isolation, isolation of mononuclear cells,…

2) Molecular genome and proteome analysis: the techniques are integrated in several experiments. Students will perfom Yeast-2-hybrid, Western blotting, mutagenesis, RFLP-PCR and genotyping of DNA-markers.

3) Cell culture experiments: students will learn the basic techniques concering the growth of eukaryotic cells in a LAF-cabine.