Separation and purification of biomolecules

Course Code :1037FBDBIC
Study domain:Biochemistry
Academic year:2017-2018
Semester:2nd semester
Sequentiality:
Contact hours:25
Credits:3
Study load (hours):84
Contract restrictions: Exam contract not possible
Language of instruction:Dutch
Exam period:exam in the 2nd semester
Lecturer(s)Xaveer Van Ostade

3. Course contents *

*cell culture tecniques: difference between cell lines and primary cells, different types of cell lines, maintenance of cell cultures

*sample preparation: preparation of cell lysates and tissue homogenization, treatment and precipitation of biomolecules

*separation techniques: different methods for centrifugation, chromatography and electrophoresis