Biochemical Separation Technology Theory
This course deals with the industrial application of unit operations in industrial biotechnology, environmental technology and the food industry. Besides important biochemical unit operations such as cell homogenisation, the separation processes adsorption, chromatography, precipitation, crystallization, membrane techniques (reverse osmosis, micro and ultrafiltration and dialysis), distillation and extraction, and their interaction with biological/biochemical factors are addressed. In addition, the mechanical and physical separation techniques filtration, sedimentation and centrifugation are applied to biological fluids. In addition to describing the set-ups used, attention is paid to the design of the biochemical unit operations.
In the course, the current trend of in-situ process recovery, in which bioreaction and separation continue simultaneously, will be discussed in more detail.
Project Biochemical separation techniques
For a specific separation problem, the appropriate laboratory experiments are carried out in group and the biological characteristic parameters are determined. By using the design parameters of the manufacturer, the most suitable industrial equipment is selected. All this is presented in a report and presented in a presentation.
The student will perform the necessary measurements and calculations for the design of the following biochemical unit operations: centrifugation, filtration, sedimentation, adsorption, liquid extraction, solid/liquid extraction, drying, chromatography, membrane filtration, precipitation, crystallisation, grinding, cell homogenisation.