Research team expertise

I study electron conduction in cable bacteria.

Technique

Raman, Cryo-EM, ToF-SIMS

Users

Researchers

Keywords

Bacteria, Sediments, Microbiology

Mass spectrometry, metabolomics and proteomics method development on new biology of cable bacteria

Technique

Proteomics, Metabolomics, Python programming

Users

Academic researchers

Keywords

Mass spectrometry, Proteomics, Metabolomics

To determine electric interactions between cable bacteria and associated bacteria, light microscopy and more advanced microscopy types such as Electron- and Raman microscopy will be used combined with molecular techniques such as isolation of bacteria, DNA and RNA followed by next-generation sequencing and bioinformatics analyses of this data to determine presence of electroactivity and proteins/genes associated with extra-cellular electron exchange. Cable bacteria and their environment will be manipulated during these experiments on microscopy-sized microcosms. Sampling trips for sediment collection and determination of presence/absence of cable bacteria and electroactivity will be determined through microsensor profiling for O2, pH, EP and H2S (both in the field and in the lab).

Technique

Light- Electron- and Raman microscopy are used to observe and follow the relationship between cable bacteria and their bacterial associates in fine detail. Molecular techniques will be used for isolating bacteria, and extracting, preparing DNA/RNA for sequencing. Bioinformatics will be used to determine what happens in the DNA/RNA.

Users

My work is located in the Electromicrobiology niche with a focus on interactions between species in an ecological (natural) setting. For that a wide array of techniques is often used.

Keywords

Molecular techniques, Cultivation, Microscopy

marine microbiology and ecology biogeochemistry oceanography climate change and global change citizen science

Technique

Microscopic imaging of microorganisms (classical, epifluorescence, SEM, AFM) Raman spectroscopy van microorganismen Moleculaire biologie van mariene microorganismen Analytical chemistry of surface waters (nutrients, metals, pH, CO2 en alkalinity) Solid phase analysis of aquatic sediments Microsensor profiling of aquatic sediments (O2, pH, H2S, EP) Biogeochemical modelling of aquatic sediments

Users

We provide a services in microscopic imaging of microbes and other biological organisms (classical, epifluorescence, scanning electron, atomic force) and analytical chemistry (microsensor profiling, porewater and solid phase analysis) to colleague scientists and industrial end-users.

Keywords

Microbiology, Microbial electricity, Marine biology, Climate change, Biogeochemistry

Focussing on the biogeochemical cycles of carbon, iron and sulfur, I study the past and present ocean, using a research approach that combines field work and laboratory incubations with numerical modeling at the local, regional and global scales. Specific topics are the importance of burrowing fauna for cycling of carbon, iron and sulfur in the Earth System, spatial patterns of redox conditions and nutrient availability in low-oxygen oceans, and the impact of anthropogenic sediment disturbances on the coastal carbon cycle. Through this work I aim to improve our understanding of the Earth’s evolution and to provide new insights into the links between life and its geochemical environment.

Technique

Chemical measurements, radio-isotope tracers, numerical modelling.

Users

policy, scientists, ...

Keywords

Theoretical study, Ecosystem function, Experimental study