Equipment & Techniques

Molecular biological techniques (RT-qPCR, Western blotting, ELISA, immunohistochemistry)

Genotyping (PCR and agarose electrophoresis)

In vitro gastrointestinal contractility (organ bath)

In vitro gastrointestinal peristalsis (adapted Trendelenburg assay)

In vivo gastrointestinal motility (gastric emptying, GI transit, Evans Blue, beads)

Visceromotor response (VMR) to colorectal distension

In vitro afferent nerve recording

In vivo portal blood flow

In vitro intrahepatic vascular resistance (liver perfusion, organ bath)

Library prepping for next-generation sequencing technologies:

  • Targeted isoform sequencing (PacBio, Nanopore)
  • 16s rRNA gene sequencing (Illumina)
  • Shotgun sequencing (metagenomics; Illumina)

Multicapillary column/ion mobility spectrometry (MCC/IMS)

Gas-chromatography-mass spectrometry (2D-GC-QTOF-MS)