The Peripheral Neuropathy Group has expertise in mapping and identifying genes, and in studying the effect of mutations on the normal functioning of genes via neuronal and non-neuronal cell lines. This information is compared to clinical, neurophysiological and neuropathological data which are essential for genotype/phenotype correlations. The construction of transgenic models is complementary to the clinical and cellular research, and enables to unravel the pathomechanisms for sensory and/or motor neuropathies. We have further expertise in identifying genes through differential suppressive subtraction hybridisation in primary motor and sensory neurons, construction of cDNA libraries, identification of genomic and protein structures, and gene expression in neuronal and non-neuronal cells, including Schwann cells. We make use of transient and stable transformations, but also of the Crispr/Cas9 genome editing technology. In recent years we gained knowledge with microscopy technologies and the phenotyping of mouse and Drosophila models for peripheral neuropathies.
TechniqueTechnologies used in molecular genetics and cell biology.
UsersPhysicians and neuromuscular centers (national and international), that have access to families and patients with disorders of the peripheral nervous system.
Cell biology, Neuroscience, Molecular genetics, Human genetics, Nervous systems, Biotechnology