Research team

Expertise

The interfaculty research group ‘Cell Biology and Histology’ has the responsibility to enlarge the core facility ‘Biomedical microscopic imaging’ and to support the community of the university concerning microscopic imaging. Available services/expertise: - scientific and technical support in setting up new experiments belonging to the expertise of the core facility, and aid with interpretation of the results - technical and scientific support of researchers and students at the different microscopy systems of the core facility, including image recording, -processing and -analysing

Identification of functional molecular pathways in the pulmonary neuroepithelial body micro-environment: an initial gene expression analysis 01/01/2011 - 31/12/2012

Abstract

A 'whole mouse genome microarray' analysis will be performed to compare the gene expression patterns of pulmonary neuroepithelial bodies (NEBs) and control airway epithelium. This approach may characterise ion channels, molecular receptors and signalling transduction pathways that make the NEB micro-environment unique. The 'unbiased' approach should allow for more focussed functional studies of these complex sensory airway receptors.

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In situ analysis of the interactions between pulmonary neuroepithelial bodies and a population of stem cell-like airway epithelial cells: a possible link with small cell lung carcinogenesis. 01/01/2008 - 31/12/2011

Abstract

For the planned studies, the mouse was chosen as a model, because we have been able to gather for this species a considerable amount of morphological and neurochemical data related to pulmonary NEBs and the associated vCE cells, and of course also because of the additional possibilities that are created for the use of transgenic and knock-out (KO) mouse models. Also, we will be able to use the in situ mouse lung-slice model that was recently developed in our lab (Pintelon et al. 2005), and which will provide unique possibilities for the proposed project via molecular live cdl imaging. Finally, it is interesting to realise that SCLC mouse models (and all other relevant genetic mouse models) can easily be combined with this lung-slice model.

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Influence of nicotine and related molecules on pulmonary neuroepithelial bodies: a new approach using confocal live cell imaging. 01/03/2006 - 31/12/2007

Abstract

Direct effects of cholinergic agonists on neuroepithelial bodies (NEBs) in mouse lungs will be studied in an in vitro model using confocal fluorescent live cell imaging. We will try to identify the involved nicotine ACh receptors on NEBs and their complex innervation by pharmacological, physiological and morphological approaches. Long-term effects of nicotine on NEBs will be studied in vitro, using lung-slice cultures, and in vivo, by 'whole-body exposure'.

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Is glutamate a neurotransmitter in nerve terminals in the lung? Possible link with neuroepithelial bodies and other airway receptors. 01/01/2003 - 30/09/2003

Abstract

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    Functional morphology of pulmonary neuroepithelial bodies in the rat. A study using neuronal tracing, immuno- and hybridization-cytochemistry, cell cultures and RT-PCR. 01/01/2001 - 31/12/2002

    Abstract

    The complex innervation pattern of pulmonary neuroepithelial bodies (NEBs) will be analysed in detail to validate existing culture models of NEBs. Localization and immunocytochemical coding of neurons involved in the innervation of NEBs and localization of receptors for transmitters will be investigated in normal and selectively denervated rats. Co-cultures of NEB cells and neurons involved in their innervation may represent a better model for the in vivo situation.

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      Functional morphology of pulmonary neuroepithelial bodies in the rat. A study using neuronal tracing, immuno- and hybridization-cytochemistry, cell cultures and RT-PCR. 01/01/1999 - 31/12/2000

      Abstract

      The complex innervation pattern of pulmonary neuroepithelial bodies (NEBs) will be analysed in detail to validate existing culture models of NEBs. Localization and immunocytochemical coding of neurons involved in the innervation of NEBs and localization of receptors for transmitters will be investigated in normal and selectively denervated rats. Co-cultures of NEB cells and neurons involved in their innervation may represent a better model for the in vivo situation.

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      Research team(s)

        01/10/1998 - 30/09/1999

        Abstract

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