Acute necrotising pancreatitis is a severe disorder with potentially life-threatening complications. The most important gastrointestinal complications are ileus and bacterial overgrowth. The pathogenesis of the pancreatitis-induced motility disturbances is largely unknown. The aim of our study is to establish the presence of motility disturbances in a murine modelof pancreatitis and to tind out which factors play a role in their pathogenesis. First, we will develop a pancreatitis model in Swiss mice by using a CDE-diet ( choline-deticient ethionine-supplemented). Secondly, gastric emptying and intestinal transit will be measured after intragastric injection of Evans blue. In this model we will investigate the role of nitric oxide (NO), adrenergic neurones, sensory afferent neurones, prostaglandines, opioids, cytokines and oxidative stress. In parallel in vitro organ bath experiments will be performed on smooth muscle strips of the gastric fundus and ileum.