Research team

Becton Dickinson FACSAria II: highspeed, fixed alignment benchtop cell sorter platform. 19/12/2008 - 18/12/2013

Abstract

Current project concerns a new generation, user-friendly, high-speed and compact flow cytometric cell sorter, capable of sorting different cell populations simultaneously, based on their intrinsic characteristics. This warrants further biological, molecular-biological and immunological research of sorted and purified cells.

Researcher(s)

Research team(s)

    Development of an integrated strategy for the control of the allergens problem in the Belgian food and catering business. (ALLERRISK - second phase) 15/12/2008 - 31/01/2011

    Abstract

    The main objective of this project is to develop for selected allergens such an integrated strategy. In such a strategy, quantitative analysis of the target allergen plays a central role. This project will result in an integrated approach for the allergens soy and hazelnut and can be applied to other allergens in the future so that the government and producers will have the tools to control the legislation concerning allergens in a relable way.

    Researcher(s)

    Research team(s)

      Influence of therapy on T cell signal transduction in Th1 and Th2 type diseases. 01/10/2008 - 30/09/2010

      Abstract

      The aim of the project is to study the role of intracellular signalling molecules in T helper cell differentiation in patients with rheumatoid arthritis (as a model of T helper 1-mediated disease) and wasp venom allergy (Th2). Relevant signalling molecules include STAT4, STAT5, STAT6 and p38 MAPK. Using a cell-based technique, the effect of stimuli from dendritic cells and regulatory T cells on the activation of cell signalling is studied, as well as the effect of therapy that is known to induce a clinically "tolerant" state.

      Researcher(s)

      Research team(s)

        Basophil activation - New applications in nutritional allergy (banana) - Flow cytometry: a new instrument for the management of food allergy. 01/06/2007 - 31/05/2009

        Abstract

        The primary objective of the project is to investigate and validate the Basophil activation test in the diagnosis of class II food allergy and to identify patients with definite food allergies. Furthermore, the technique will be applied to discriminate between clinically relevant and irrelevant sIgE antibody results.

        Researcher(s)

        Research team(s)

          Development of an integrated strategy for the control of the allergens problem in the Belgian food and catering business. (ALLERRISK) 15/12/2006 - 31/01/2009

          Abstract

          The main objective of this project is to develop for selected allergens such an integrated strategy. In such a strategy, quantitative analysis of the target allergen plays a central role. This project will result in an integrated approach for the allergens soy and hazelnut and can be applied to other allergens in the future so that the government and producers will have the tools to control the legislation concerning allergens in a relable way.

          Researcher(s)

          Research team(s)

            Influence of therapy on T cell signal transduction in Th1 en Th2 type diseases. 01/10/2006 - 30/09/2008

            Abstract

            Researcher(s)

            Research team(s)

              Influence of antigen specific immunotherapy on the intracellular signal transduction of basophils. 01/10/2006 - 30/09/2007

              Abstract

              The purpose of this project is to obtain further insights in the early appearing tolerance mechanisms of antigen specific immunotherapy in wasp anaphylaxis. For this purpose the intracellular signal transduction of basophils of patients treated with wasp venom immunotherapy will be studied.

              Researcher(s)

              Research team(s)

                Study of in vivo and in vitro modulation of dendritic cells in Th1 (rheumatoid arthritis) and Th2 (venom allergy) mediated diseases. Influence of anti-TNF and immunotherapy. 01/01/2006 - 31/12/2009

                Abstract

                Researcher(s)

                Research team(s)

                  Evaluation of immunoregulatory mechanisms in lymphocytes and basophils in patients with hymenoptera venom anaphylaxis : effects of venom immunotherapy. 01/10/2003 - 31/12/2005

                  Abstract

                  Venom immunotherapy has proven to be very effective in hymenoptera venom anaphylaxis. However, there are no good parameters to indicate when to stop venom immunotherapy. The objectives of this work are to assess the relationship of the basophil activation test to history, skin test and venom-specific IgE and to address the time course of basophil activation to venom during venom immunotherapy and the possible utility of basophil activation test to determine the duration of therapy.

                  Researcher(s)

                  Research team(s)

                    01/06/2002 - 31/12/2004

                    Abstract

                    Researcher(s)

                    Research team(s)

                      Study of cord blood monocytes in neonates of atopic and non-atopic parents. 01/06/2002 - 31/12/2002

                      Abstract

                      It is postulated that cord blood monocytes from neonates of atopic parents have different features than monocytes from newborns ofnon-atopic parents. Our hypothesis will be evaluated at two levels. After incubation with antigens, the secretion of cytokins and PGE2 by cord blood monocytes will be assessed. Further the influence of primed monocytes on the lymphocytic immune response will be determined by evaluating T -cell proliferation and production of cytokines by T -lymphocytes.

                      Researcher(s)

                      Research team(s)

                        Allowance for research equipment : flow cytometer. 01/01/2002 - 31/12/2002

                        Abstract

                        The flow cytometer is an indispensable apparatus for the analysis of cell membrane characteristics (blood-, umbilical-cartilage cells) and for the detection of intracellular mediators (e.g. cytokines) in these cells.

                        Researcher(s)

                        Research team(s)

                          The role of perinatal exposure to allergens and pollutants in the genesis of asthma, allergic rhinitis, and atopic eczema. 01/01/2001 - 31/12/2004

                          Abstract

                          Introduction: From 1997 on, a research project on the effect of risk factors before and after birth (perinatal) on the occurrence of asthma and allergies in children has started. The project is called PIPO-study which stands for Prospective study on the Influence of Perinatal factors on the Occurrence of asthma and allergies in newborns. Pregnant women are asked to participate and their children will be followed until at least the age of 4. Meanwhile approximately 800 subjects participate and recruitment will continue until May 2001, resulting in approximately 1,200 children included in the study. Research questions of the PIPO-study: 1) What determinants during the perinatal period detemine the occurrence of asthma and other atopic diseases (rhinitis, atopical eczema)? 2) What determinants (effect modifiers) determine the degree of association between exposure to common allergens (house dust mite, cat dander) and the occurrence of asthma and allergy? 3) How does the immunological reaction to these allergens develop over time and what is its relationship to clinical symptoms? 4) Are the determinants for the occurrence of asthma and allergy avoidable? Aim of the project: Performance of the follow-up of the children of the PIPO-study at the age of 4 years, since the study population has already mainly been formed. Working plan: During the project two dust samples have been taken: at 5 months pregnancy and 3 months after birth. The amount of house dust mite allergens in these dust samples will be determined. Moreover, 2 extended questionnaires are being taken, in which several risk factors will be established (familial predisposition, smoking behaviour, nutrition, indoor factors,'). Especially determinants before, around and after birth (peri-natal) will be asked for. During the first year, the parents record allergic and respiratory symptoms once a week. The physician of family wellfare clinic (`Kind&Gezin') or the general practitioner will be asked to fill in a medical questionnaire 4 times a year. At the age of one, when the parents give permission, all children will be physically examined by a research physician and a blood sample will be taken for an evaluation of the allergy status. Since asthma and allergy especially occur at later age, the further follow-up of these children is very important. This project will therefore carry out this follow-up. During the entire research period, data of all children on changes in environmental determinants and the occurrence of asthma and atopic diseases will be collected by postal questionnaires, which are filled in by their parents. Subsequently an extended examination will take place at the age of 4. This will be carried out by questionnaire, physical examination and a blood sample for determination of total and specific allergic markers in the blood. Results will published in a report and will be submitted to international journals.

                          Researcher(s)

                          Research team(s)

                            01/01/2001 - 31/05/2002

                            Abstract

                            Researcher(s)

                            Research team(s)

                              Influence of immune complexes on chondrocyte function in rheumatoid arthritis 01/01/2000 - 31/12/2002

                              Abstract

                              In this project, the influence of immune complexes on chondrocyte functions will be studied in rheumatoid arthritis. Immune complexes are present in serum, synovial fluid and superficial layers of cartilage of rheumatoid arthritis patients. Experimental data in animals suggest that immune complexes provoque cartilage destruction. However data in man are lacking. Parameters of chondrocyte function that will be investigated include chondrocyte growth, production of inflammatory mediators, necrosis and apoptosis.

                              Researcher(s)

                              Research team(s)

                                Study of cord blood monocytes in neonates of atopic and non-atopic parents. 01/01/2000 - 31/05/2002

                                Abstract

                                It is postulated that cord blood monocytes from neonates of atopic parents have different features than monocytes from newborns of non-atopic parents. Our hypothesis will be evaluated at two levels. After incubation with antigens, the secretion of cytokins and PGE2 by cord blood monocytes will be assessed. Further the influence of primed monocytes on the lymphocytic immune response will be determined by evaluating T-cell proliferation and production of cytokines by T-lymphocytes.

                                Researcher(s)

                                Research team(s)

                                  Environment and allergy, environment and autoimmune diseases 01/01/2000 - 30/06/2000

                                  Abstract

                                  In this paper the actual knowledge about environment and allergy is discussed: multiple allergens can cause allergy of the respiratory system and the individuals is exposed via different possible sites. Household allergens, introduced by the patient himself are very important, next to professional exposures and exposure via the "macro"environment. For autoimmune diseases, the situation is less clear cut: there are a number of well known examples leading to exacerbation or induction of generalised autoimmune diseases. Examples are ultraviolet light and systemic lupus erythematodes disseminatus (SLE), estrogens and other drugs and SLE. The toxic oil syndrome and eosinophilic fasciitis are associated with oral intake of toxic oil and L-tryptophan. A number of substances (e.g. PVC) can induce systemic sclerosis.

                                  Researcher(s)

                                  Research team(s)

                                    Thrombin generation and thrombosis - importance of genetic and immune phenomena, value of pharmacological interventions. 21/12/1998 - 22/12/2001

                                    Abstract

                                    This project will study 4 main topics : 1. autoantibodies in thrombotic events; 2. impact of fibronolytics on thrombin generation, contact and complement activation; 3. effect of antiplatelet and lipid-lowering drugs and nutrition supplementation on thrombin generation; 4. genetic aspects of thrombophilia.

                                    Researcher(s)

                                    Research team(s)

                                      T-helper 1 and T-helper 2 cytokine profile during hymenoptera hyposensitization. 01/11/1997 - 30/10/1999

                                      Abstract

                                      Hyposensitization with wasp venom induces a dramatic decrease of lymphocyte stimulation with the venom, we will evaluate the T-helper 1 and T-helper 2 cytokine profile in order to unravel the mechanisms of the short term and long term immunological effects.

                                      Researcher(s)

                                      Research team(s)

                                        The role of perinatal exposure to allergens and polluents in the genesis of asthma, allergic rhinitis and atopic eczema. 01/01/1997 - 31/12/1999

                                        Abstract

                                        In a cohort study 2000 newborns will be enrolled starting 6 months before birth. By means of a questionnaire, dust sampling and biological monitoring, data on exposure to determinants of asthma, allergic rhinitis and atopic eczema will be assessed. The incidence of these diseases will be registred at least until 2 years of follow-up are completed. In a nested case-referent study the occurrence of asthma, allergic rhinitis and atopic eczema will be studied in detail as a function of exposure to allergens, with special emphasis on the potential effect modification by pollutants.

                                        Researcher(s)

                                        Research team(s)

                                          Study of cord blood lymphocytes as a prediction of atopic disorders (asthma and atopic dermatitis). 01/01/1996 - 31/12/1999

                                          Abstract

                                          The aim of the project is to study cord blood lymphocytes of an unselected group of neonates; the children have to be born after an uncomplicated pregnancy and a normal birth. All children will be followed during the first two years of life with special attention to the development of asthma and atopic dermatitis. The purpose is to look if cord blood lymphocytes of subsequently atopic children have other features than cord blood lymphocytes of normal children.

                                          Researcher(s)

                                          Research team(s)

                                            Study of cord blood lymphocytes as a prediction of atopic disorders (asthma and atopic dermatitis). 01/01/1996 - 31/12/1996

                                            Abstract

                                            The aim of the project is to study cord blood lymphocytes of an unselected group of neonates; the children have to be born after an uncomplicated pregnancy and a normal birth. All children will be followed during the first two years of life with special attention to the development of asthma and atopic dermatitis. The purpose is to look if cord blood lymphocytes of subsequently atopic children have other features than cord blood lymphocytes of normal children.

                                            Researcher(s)

                                            Research team(s)

                                              Activation status of neutrophils in airways in patients with chronic obstructive lung disease (cold) and asthma. 01/01/1995 - 31/12/1996

                                              Abstract

                                              Neutrophils isolated from periferal blood or broncho-alveolar lavage will be studied before and after treatment with inhaled corticosteroids and/or anti-oxidants (N-acetylcysteine). Membrane adhesion molecules, chemotaxis, superoxide production, actin polymerisation status and a number of cytokines will be evaluated in order to determine the role of the neutrophils in this process.

                                              Researcher(s)

                                              Research team(s)

                                                Leucocyte adhesion and interaction with endothelium and cartilage in rheumatoid arthritis 01/10/1992 - 30/09/1996

                                                Abstract

                                                In this project on rheumatoid arhtritis, an important initial step in the inflammatory proces, i.e. the adhesion of leukocytes to endothelium, is studied. Adhesive properties of neutrophils and eosinophils are compared. Furthermore, on cartilage level, the adhesion and interaction of granulocytes and chondrocytes is investigated.

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                                                Research team(s)