Equipment and Techniques

        Equipment:

  • Flow cytometers; BD FACSymphonyTM A1, BD Facs CAliburTM and BD Facs CantoTM II (BD Biosciences)
  • Cell culture facility
  • Phadia 250 (Thermo Fisher)
  • Lux Scan 10K-A microarray scanner (Capital Bio)
  • Äkta start protein purification system (GE Healthcare Life Sciences)
  • Alpha 1-4 LD plus freeze dryer (Christ)
  • SavantTM SPD 111 SpeedVacTM concentrator (Thermo Scientific)
  • BX43 upright microscope with U-GAN for Gout diagnostic (Olympus)
  • MultiscanTM microplate photometer (Thermo Scientific)
  • CytospinTM 4 cytocentrifuge (Thermo Scientific)
  • MoxiTM Z automated cellcounter (Orflo)

        Techniques:

  • Basophil Activation Test (BAT)
  • Direct and passive Mast Cell Activation Test (dMAT and pMAT)
  • CD34 derived Mast Cell cultures
  • Westernblot
  • ELISA
  • Cytometric Bead Array (CBA)
  • Immunofluorescence and live cell immaging