Flow cytometry is a technique used to examine and measure the expression of intracellular molecules and cell surface, as well as to define and characterize distinct cell populations. It can also be used to determine the cell size and volume to assess the purity of subpopulations.
This method enables the simultaneous multi-parameter analysis of individual cells. Flow cytometry measures the fluorescence intensity generated by by fluorescently labeled antibodies, which are used to detect proteins or ligands bound to cells.
