Projects

Maternale obesitas en "fetal programming": de gevolgen voor de voortplantingsfysiologie van de nakomelingen.
01/10/2018 - 30/09/2022

Abstract

A disturbed maternal metabolism like in obesity or type II diabetes has clearly been associated with disappointing fertility. We extensively showed that such metabolic disorders have direct effects on the micro-environment of the growing and maturing oocyte, ultimately leading to reduced oocyte and embryo quality. Obesity is a global health threatening problem and recent studies indicated that maternal obesity may result in significant health issues in the offspring. More in depth mechanistic research clearly pointed out the importance of uterine programming in early pregnancy. It is not known however whether the metabolic status of obesity as such and/or potential direct effects of the typical fat rich western type diet are responsible for these observations. Based on the epidemiological relevance of obesity and hypercholesterolemia we hypothesize that obesity or an obesogenic diet of the mother around conception or during the entire pregnancy will alter the micro-environment of the growing embryo and fetus. This will change uterine programming ultimately leading to compromised offspring's health and reproductive physiology. To systematically investigate this hypothesis, we will feed female LDLR knock-out mice (LDLR-/-) an obesogenic diet A) several weeks before conception resulting in maternal obesity at conception or B) solely around conception or C) throughout the entire pregnancy. The offspring will be cross-fostered upon birth and will be used to study the general health of the offspring, the ovarian follicular reserve and the process of folliculo- and oogenesis, the offspring's pre-implantation embryo physiology and gene expression pattern and the receptivity of the offspring's uterus to support full pregnancy resulting in healthy offspring. By using this strategic experimental model we will be able to find the most sensitive window during pregnancy for uterine programming of reproduction, and it allows us to study the effects on every specific step on reproductive functioning. We believe that this project proposal may significantly contribute to the concept of "Developmental Origin of Health and Fertility" by further spreading the knowledge that epigenetic effects of maternal metabolism and diet may jeopardize health but also fertility in the offspring.

Researcher(s)

Promotor: Leroy Jo
Co-promotor: De Keulenaer Gilles
Co-promotor: Marei Waleed

Research team(s)

Veterinary physiology and biochemistry

Can antioxidants ameliorate the development and quality of embryos under maternal metabolic stress conditions?
01/04/2018 - 31/03/2019

Abstract

Subfertility is a global public health issue. The main causes of subfertility are metabolic diseases such as obesity or type 2 diabetes. Also in farm animals, the link between metabolic stress and subfertility has been acknowledged. Maternal metabolic disorders are associated with upregulated lipolysis leading to a disturbed metabolic profile in the blood (increased non-esterified fatty acids (NEFAs), ketones, altered glucose and insulin). Using the cow as a model, we showed that this altered metabolic profile is reflected in the follicular and oviductal environment in which the oocyte and embryo develop, subsequently altering oviductal functions and jeopardizing oocyte and embryo development and quality. More mechanistic research revealed that increased oxidative stress is one of the key underlying pathways associated with metabolic stress at oocyte and embryo level. Therefore, antioxidants may play an important role in alleviating oxidative stress in metabolically compromised embryos, hereby ameliorating embryo developmental competence and quality and thus fertility. We already showed that circulating antioxidants are reflected in the oocyte's micro-environment and can be altered by the diet. Little is known about the antioxidative environment of the pre-implantation embryo (in the oviduct) and whether these antioxidants can influence the development and quality of metabolically stressed embryos. As such, we hypothesize that dietary antioxidant supplementation in metabolically compromised mothers can change oviductal concentrations of antioxidants hereby changing oviductal cell functions and ameliorating embryo developmental competence and quality. Therefore, the objective of the research proposed here is to culture in vitro derived bovine zygotes in the oviducts of metabolically stressed ewes supplemented with antioxidants and investigate the antioxidative status of the oviductal fluid, the characteristics of the oviduct at the transcriptomic level and the embryo developmental competence and quality. Enhancing the oviductal environment to better support early embryo development is crucial for the embryos to establish successful pregnancy later in development. These results can contribute to drastically reduced medical costs for subfertility treatments. In addition, they may tackle the subfertility problem in high yielding dairy cows hereby improving sustainable and profitable farming.

Researcher(s)

Promotor: De Bie Jessie

Research team(s)

Veterinary physiology and biochemistry

The first embryo-dependent and -independent programming of endometrial function in early pregnancy: a bovine model.
01/03/2018 - 31/08/2018

Abstract

Subfertility represents a major problem in domestic animals and humans. In cattle but also in women, up to 40% of total embryonic losses occur between days 7 and 16 of pregnancy, indicating that early embryonic mortality is a major cause of reproductive failure. A finely tuned synchrony between the competent embryo and a receptive endometrium is the key for optimal embryo development and the establishment of a successful pregnancy. Several studies have indicated the existence of complex paracrine and endocrine in vivo communication between early embryo and the maternal tract in mammals. However, so far there is no evidence that the embryo significantly interacts with the endometrium before elongation in the cow (begins 13 days post-mating) or before real implantation in human (day 10-17), while most of the early embryonic losses happen at that time. To understand the pathways of early pregnancy loss we have to elucidate the physiological molecular and biochemical processes underpinning and regulating the earliest maternal-embryonic cross talk upon the moment of embryo arrival in the uterus. In the present project, using the cow as a proven research model to study the very first developmental stages of the pre-implantation embryo, our central aim is to study the embryo/conceptus and endocrine programming of the endometrium to support pregnancy success. In the first part of the PhD research (Milestones 1 and 2, conducted at the University of São Paulo) we hypothesized that exposure to an embryo changes the abundance of specific transcripts and the biochemical composition of the uterine fluid in the cranial region of the pregnant uterine horn. As such an effect is likely to be very local in nature, we developed an in vivo model that allowed sampling the endometrium closer to the embryo (Sponchiado et al., 2017). To limit the use of experimental animals, in the second part of the PhD research (Milestone 3), we propose to use an innovative in vitro co-culture system to investigate the nature of the specific interaction between bovine embryos and endometrial epithelial cells and how this ultimately may affect early embryo quality. This part of the project will be conducted at the University of Antwerp. The proposed combination of in vivo and in vitro systems with state-of-the art analytical tools of transcriptome and metabolomic research should reveal a plethora of candidate genes and bioactive molecules to understand the pathways of the very first embryo-maternal dialogue. The very first communication between the mother and her embryo is undoubtedly one of the most exciting processes in reproductive biology. The knowledge of these mechanisms should help to understand the problem of early embryo mortality and pregnancy loss leading to disappointing fertility results. Only then it will be possible to build on preventive and even therapeutic measures impacting in pregnancy success both in human and agricultural application.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

Development of strategic preconception care to improve maternal fertility and protect offspring's health in obese individuals: steps towards evidence based clinical advice.
01/01/2018 - 31/12/2021

Abstract

Obesity is becoming a global threat, reducing mother's health and reproductive capacities and affecting the offspring's health. Clear preconception care guidelines for obese future mothers are completely lacking. Clinical studies are flawed and more fundamental studies in basic animal models are very scarce. The importance of preconception weight loss on reproduction and baby's health is heavily debated and never been investigated in detail. This research project aims to propose strategically designed animal research models to provide relevant scientific evidence for effective and sustainable preconception care advices to obese women planning for pregnancy. To do so, we will assess the impact of preconception weight loss, diet normalization, increased physical activity, omega-3 rich diet or the combination thereof in obese outbred mother mice on four distinct major outcome parameters: 1) improvement of mother's metabolic profile before conception in relation to the observed weight loss; 2) mother's own fertility success; 3) postnatal health of the offspring and 4) offspring's reproductive physiology. Effective guidelines for preconception care lifestyle interventions in obese patients will drastically lower the (public) health care cost associated with assisted reproduction and should maximally safeguard the health of the baby. The data generated will be the basis for awareness and education programs aiming at improved maternal health at the time of conception.

Researcher(s)

Promotor: Leroy Jo
Co-promotor: De Neubourg Diane
Fellow: Smits Anouk

Research team(s)

Veterinary physiology and biochemistry

Comparison of nano-injection and aquatic exposure in fish embryo tests: can differences in toxicity be explained by accumulation kinetics?
01/04/2017 - 31/03/2018

Abstract

Nano-injection is a promising alternative exposure route for toxicological research using fish embryos. We recently started applying nano-injections and observed differences in toxicity compared to exposure via water. While it is plausible that uptake and biotransformation play a role in these differences, this has never been investigated. The aim of this project is to characterize and understand differences in toxicity following nano-injection compared to aquatic exposure. We will compare accumulation, biotransformation, molecular events and physiological effects after exposure of zebrafish embryos to the same chemical via both exposure routes. This information is essential to evaluate the applicability of this promising alternative exposure route in (eco)toxicology.

Researcher(s)

Promotor: Vergauwen Lucia

Research team(s)

Veterinary physiology and biochemistry

Mitochondrial and endoplasmic reticulum stress in oocytes and embryos as treatment targets for infertile patients with metabolic diseases.
01/04/2017 - 31/03/2018

Abstract

Oxidative stress, mitochondrial and endoplasmic reticulum (ER) stress have been recently postulated as main drivers of altered pathways in the oocyte linking maternal metabolic disorders to low fertility outcomes. Upregulated lipolysis, commonly associated with obesity and other metabolic disorders, increases the concentrations of lipotoxic fatty acids in the follicular fluid, which were shown to have a direct detrimental impact on oocyte quality and subsequent embryo development. Oocyte maturation involves complex nuclear, cytoplasmic, and molecular changes that determines its developmental competence. In human IVF clinics, oocytes are usually collected after hormonal stimulation and thus after exposure to metabolic stress during maturation in the follicle in affected mothers. It is not known if the deterioration in developmental competence of these metabolically-compromised oocytes can be rescued by alleviating mitochondrial and endoplasmic stress during or after fertilization, or should the stress be prevented during maturation. This project aims to test sensitive windows for preventative or reparative measures that may improve in vitro embryo production from metabolically-compromised oocytes, and examine the intrinsic quality of the embryos produced after treatment. A well-established bovine in vitro model will be used to generate metabolically-compromised oocytes by exposure to elevated pathophysiological levels of palmitic acid. MitoQ, a highly efficient mitochondria-targeted antioxidant, and Sirolimus, a specific ER-stress inhibitor, will be used to alleviate cellular stress either during fertilization or embryo culture, or during maturation. Embryo development will be recorded and blastocysts quality will be assessed by examining their metabolism, cell proliferation, cell lineage, DNA damage, markers for cellular stress and apoptosis. RNA-seq will be used to detect any persistent alterations in the transcriptomic profile. These studies will help defining windows of sensitivity and efficiency of targeting intracellular stress as a treatment option for infertile patients suffering from metabolic diseases.

Researcher(s)

Promotor: Marei Waleed

Research team(s)

Veterinary physiology and biochemistry

reproduction as the cornerstone for sustainability in the dairy business: feeding for optimal fertility
01/01/2017 - 31/12/2017

Abstract

This project focuses on how strategically applied anti-oxidants in the ratio can improve dairy cow fertility through an improved oocyte and embryo quality. The research will focus on very specific parameters of the oocyte's and embryo's micro-environment and of the quality of the female gamete.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

Mother-to-embryo impact of maternal metabolic diseases: nutrient sensing pathways drive developmental and differentiation programs in the bovine embryo.
01/01/2017 - 31/08/2017

Abstract

Metabolic disorders have a profound effect on many aspects of reproduction. A growing number of animal in vivo studies indicate that maternal metabolic diseases not only jeopardize embryo implantation, but also program the well-being of the fetus. Awareness grows on the connection between maternal metabolic health, embryo development and blastocyst cell differentiation. This link is conceptualized as the developmental programming hypothesis whereby environmental influences during critical periods of developmental plasticity can elicit effects from mother-toembryo and even from mother-to-child. During the critical first week of development, the embryo has a remarkable metabolic flexibility that allows to grow and develop, even under suboptimal nutrient conditions. However, altered metabolic strategies in the early embryo might come at a cost. Stem cell studies continue to elicit the intriguing link between nutrient abundance and processes that drive cell differentiation. In this context, we propose that the early bovine embryo, developing in a metabolically compromised individual, will contend a severely impaired capacity to support a normal maternal-embryonic crosstalk upon arrival in the uterus. Thus far, no studies enlighten the link between nutrient sensing paths and blastocyst cell lineage differentiation, although understanding of the underlying pathogenesis will be indispensable for designing effective treatments.

Research team(s)

Veterinary physiology and biochemistry

Mitochondria take centre stage: pathways to reduced oocyte quality and opportunities for curative strategies under maternal metabolic stress conditions.
01/10/2016 - 30/09/2019

Abstract

Infertility is a major socio-economic problem affecting millions worldwide and is specifically linked to maternal obesity and other (diet induced) metabolic disorders. Understanding the mechanisms by which altered metabolism affect fertility is crucial for successful interventions. Mitochondria are the power house within the oocyte. Reduced somatic cell mitochondrial function occurs early in the pathogenesis of metabolic diseases. This is mainly due to the lipotoxic effects of elevated free fatty acid concentrations in blood. For the oocyte to be developmentally competent, the number and function of mitochondria should reach a certain threshold. There are several thousands of mitochondria in the mature oocyte derived from about 20 mitochondria in the germ cell. In addition to their bio-energetic roles, mitochondria are also sensors of stress. Oxidative stress and associated cellular damage elicit stress signalling between the mitochondria and the nucleus to start a protective machinery. The effects of metabolic stress on mitochondrial replication and stress responses during oocyte growth and subsequent embryo development are not known. In this project we will use in vitro and in vivo animal models to study mitochondrial functions and stress responses under maternal metabolic stress conditions in growing oocytes. Defect-based protective and rescue interventions will also be tested to investigate opportunities for curative interventions.

Researcher(s)

Promotor: Leroy Jo
Fellow: Marei Waleed

Research team(s)

Veterinary physiology and biochemistry

Maternal obesity and uterine programming: the consequences for the offspring's reproductive physiology.
01/10/2016 - 31/12/2017

Abstract

A disturbed maternal metabolism like in obesity or type II diabetes has clearly been associated with disappointing fertility. We extensively showed that such metabolic disorders have direct effects on the micro-environment of the growing and maturing oocyte, ultimately leading to reduced oocyte and embryo quality. Obesity is a global health threatening problem and recent studies indicated that maternal obesity may result in significant health issues in the offspring. More in depth mechanistic research clearly pointed out the importance of uterine programming in early pregnancy. It is not known however whether the metabolic status of obesity as such and/or potential direct effects of the typical fat rich western type diet are responsible for these observations. Based on the epidemiological relevance of obesity and hypercholesterolemia we hypothesize that obesity or an obesogenic diet of the mother around conception or during the entire pregnancy will alter the micro-environment of the growing embryo and fetus. This will change uterine programming ultimately leading to compromised offspring's health and reproductive physiology. To systematically investigate this hypothesis, we will feed female LDLR knock-out mice (LDLR-/-) an obesogenic diet A) several weeks before conception resulting in maternal obesity at conception or B) solely around conception or C) throughout the entire pregnancy. The offspring will be cross-fostered upon birth and will be used to study the general health of the offspring, the ovarian follicular reserve and the process of folliculo- and oogenesis, the offspring's pre-implantation embryo physiology and gene expression pattern and the receptivity of the offspring's uterus to support full pregnancy resulting in healthy offspring. By using this strategic experimental model we will be able to find the most sensitive window during pregnancy for uterine programming of reproduction, and it allows us to study the effects on every specific step on reproductive functioning. We believe that this project proposal may significantly contribute to the concept of "Developmental Origin of Health and Fertility" by further spreading the knowledge that epigenetic effects of maternal metabolism and diet may jeopardize health but also fertility in the offspring.

Researcher(s)

Promotor: Leroy Jo
Co-promotor: De Keulenaer Gilles
Fellow: Smits Anouk

Research team(s)

Veterinary physiology and biochemistry

Development of an alternative testing strategy for the fish early-life stage test for predicting chronic toxicity : assay validation.
01/03/2016 - 28/02/2018

Abstract

This project aims to develop an alternative testing strategy to reduce the need for fish early life-stage toxicity tests (FELS) for the assessment of chronic toxicity of chemicals to fish, using the adverse outcome pathway (AOP) framework as a guidance for assay development. Currently, the FELS test (OECD TG 210) is one of the primary testing guidelines used to estimate the chronic toxicity of chemicals to fish. Results obtained using this TG are used to support risk assessment around the world. However, important limitations of this animal test are being recognized, including (1) the high numbers of fish used, (2) the relatively low-throughput, and (3) the lack of mechanistic information. In order to comply with the 3R principles, we are developing a non-animal testing strategy which includes both in vitro tests and in vivo whole organism alternative 120 hpf (hours post fertilization) ZFET (zebrafish embryo acute toxicity test) assays. One of the main research questions of this project is to what extent an assay development approach based on the AOP framework is capable of offering a mechanistic basis for selecting assays to develop an alternative testing strategy that allows the prediction of chronic FELS toxicity. This project is a follow-up of Cefic LRI-ECO20, and aims to validate assays that were developed during that project.

Researcher(s)

Promotor: Knapen Dries
Co-promotor: Blust Ronny
Co-promotor: Vergauwen Lucia

Research team(s)

Veterinary physiology and biochemistry

An alternative testing strategy for the fish early life-stage test using the adverse outcome pathway (AOP) framework.
01/01/2016 - 31/12/2016

Abstract

This project aims to develop an alternative testing strategy to reduce the need for fish early life-stage toxicity tests (FELS) for the assessment of chronic toxicity of chemicals to fish, using the adverse outcome pathway (AOP) framework as a guidance for assay development. Currently, the FELS test (OECD TG 210) is one of the primary testing guidelines used to estimate the chronic toxicity of chemicals to fish. Results obtained using this TG are used to support risk assessment around the world. However, important limitations of this animal test are being recognized, including (1) the high numbers of fish used, (2) the relatively low-throughput, and (3) the lack of mechanistic information. In order to comply with the 3R principles, we are developing a non-animal testing strategy which includes both in vitro tests and in vivo whole organism alternative 120 hpf (hours post fertilization) ZFET (zebrafish embryo acute toxicity test) assays. One of the main research questions of this project is to what extent an assay development approach based on the AOP framework is capable of offering a mechanistic basis for selecting assays to develop an alternative testing strategy that allows the prediction of chronic FELS toxicity.

Researcher(s)

Promotor: Knapen Dries
Co-promotor: Stinckens Evelyn

Research team(s)

Veterinary physiology and biochemistry

The impact of endocrine disruption on vertebrate embryonic and larval development.
01/10/2015 - 30/09/2019

Abstract

Endocrine disruption is a major concern for the health of wildlife populations. Although many studies have shown reproductive impairment as a consequence of endocrine disruption in adult fish, knowledge of the consequences of endocrine disruption for vertebrate embryonic and larval development is scarce. The zebrafish embryo is an ideal model system to investigate the fundamental mechanisms of endocrine disruption. We will first describe the timing of the normal embryonic activation of the hormone synthesis machinery, as well as the hormone profiles, during the early stages of vertebrate development, which has never been done so far. Secondly, using well-described endocrine disrupting compounds, we will specifically disrupt these profiles and propose mechanisms linking the changed profiles to observed developmental effects. We will then validate the proposed mechanisms of toxicity using targeted disruption techniques, including knockout and morpholino knockdown. This project will offer the information that is needed for follow-up projects to develop assays to specifically assess the risk associated with exposure to different classes of EDCs on embryonic and larval development, allowing discrimination among ER and AR agonism and antagonism, as well as aromatase inhibition. Such assays would fit perfectly within both EU and USA regulatory priorities for screening and prioritizing potential EDCs.

Researcher(s)

Promotor: Knapen Dries
Co-promotor: van Nuijs Alexander
Co-promotor: Vergauwen Lucia
Fellow: Periz Stanaćev Jelena

Research team(s)

Veterinary physiology and biochemistry

High non-esterified fatty acid concentrations during preimplantation embryo development: Consequences for female fertility and offspring's health.
01/10/2015 - 11/11/2017

Abstract

To further expand on this intriguing patho-phsyiological role of elevated NEFA in the problem of subfertility, it need to be addressed whether NEFA can induce epigenetic changes and whether this affects further embryo development and postnatal health. Therefore, this research proposal concentrates on the effect of oocyte maturation under elevated NEFA conditions on DNA methylation patterns in Day 7 embryos, on further pre-implantation in vivo development and on postnatal health and growth. A state of the art in vitro embryo culture and embryo transfer protocols will be used combined with advanced molecular techniques. The integration of data on Day 7 embryo physiology, DNA methylation, further development and postnatal health could provide key metabolic information on the role of elevated NEFA concentrations in reproductive failure.

Researcher(s)

Promotor: Leroy Jo
Co-promotor: Bols Peter

Research team(s)

Veterinary physiology and biochemistry

Maternal metabolic disorders and early embryonic loss: pathways to bridge the gap between embryo quality and endometrial receptivity.
01/10/2015 - 31/08/2017

Abstract

Metabolic disorders have a profound effect on many aspects of reproduction. High rates of early embryonic loss are a major issue. Upon arrival in the uterus, the proportion of surviving embryos drops off rapidly, which points towards troubled first interactions between embryonic and endometrial tissues. Recent insights suggest that embryo energy metabolism can be involved, as nutrient-sensing pathways regulate differentiation, the latter being crucial to guarantee the first interplay between embryonic and endometrial cells. During the critical first week of development, the embryo has a remarkable metabolic plasticity that allows to grow and develop, even under suboptimal nutrient environments. At the blastocyst stage, this early metabolic adaptation may come at a cost, ultimately leading to pregnancy failure. Herein, we hypothesise that suboptimal metabolic conditions in the early environment of the embryo can impact on the differentiation signature of the resultant trophoblast cells. This might be 'sensed' by the endometrium. We also propose that a differentiated endometrium has the potency to reset and rebalance the embryonic metabolism. Embryo and endometrial 'go' or 'no-go' responses will be studied using bovine in vitro and in vivo set-ups. While embryo implantation varies among species, the initial events between trophoblast and endometrial cells are shared among mammals, which implies that crossspecies lessons can be learned from the cow.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

A functional study of the mechanisms underlying sex differentiation in zebrafish
01/02/2015 - 31/12/2015

Abstract

Several fish, amphibian and reptile species exhibit non-genetic sex determination, which is currently poorly understood. Recent advances have identified several genes and pathways involved based on expression patterns, but the functional role of these genes in sex determination and differentiation remains unclear. By endocrine disruption of this process, we will investigate the role of selected genes in sex differentiation in the zebrafish.

Researcher(s)

Promotor: Vergauwen Lucia

Research team(s)

Veterinary physiology and biochemistry

Development of a zebrafish embryo test for environmental risk assessment of pharmaceuticals with estrogenic properties.
01/01/2015 - 31/12/2018

Abstract

Pharmaceutical companies are obligated to perform an environmental risk assessment for each new drug that they launch on the market. The mandatory tests for potential endocrine disrupting compounds require a lot of time and laboratory animals. Therefore, the purpose of this study is to develop a zebrafish embryo test, which is not considered as animal test, that is capable of quickly detecting estrogenic properties of pharmaceuticals.

Researcher(s)

Promotor: Knapen Dries
Co-promotor: Van Cruchten Steven
Fellow: Michiels Ellen

Research team(s)

Veterinary physiology and biochemistry

Maternal obesity and uterine programming: the consequences for the offspring's reproductive physiology.
01/10/2014 - 08/05/2015

Abstract

A disturbed maternal metabolism like in obesity or type II diabetes has clearly been associated with disappointing fertility. We extensively showed that such metabolic disorders have direct effects on the micro-environment of the growing and maturing oocyte, ultimately leading to reduced oocyte and embryo quality. Obesity is a global health threatening problem and recent studies indicated that maternal obesity may result in significant health issues in the offspring. More in depth mechanistic research clearly pointed out the importance of uterine programming in early pregnancy. It is not known however whether the metabolic status of obesity as such and/or potential direct effects of the typical fat rich western type diet are responsible for these observations. Based on the epidemiological relevance of obesity and hypercholesterolemia we hypothesize that obesity or an obesogenic diet of the mother around conception or during the entire pregnancy will alter the micro-environment of the growing embryo and fetus. This will change uterine programming ultimately leading to compromised offspring's health and reproductive physiology. To systematically investigate this hypothesis, we will feed female LDLR knock-out mice (LDLR-/-) an obesogenic diet A) several weeks before conception resulting in maternal obesity at conception or B) solely around conception or C) throughout the entire pregnancy. The offspring will be cross-fostered upon birth and will be used to study the general health of the offspring, the ovarian follicular reserve and the process of folliculo- and oogenesis, the offspring's pre-implantation embryo physiology and gene expression pattern and the receptivity of the offspring's uterus to support full pregnancy resulting in healthy offspring. By using this strategic experimental model we will be able to find the most sensitive window during pregnancy for uterine programming of reproduction, and it allows us to study the effects on every specific step on reproductive functioning. We believe that this project proposal may significantly contribute to the concept of "Developmental Origin of Health and Fertility" by further spreading the knowledge that epigenetic effects of maternal metabolism and diet may jeopardize health but also fertility in the offspring.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

Zebrafish embryos to elucidate the role of thyroid hormones in vertebrate embryonic development.
01/02/2014 - 31/12/2014

Abstract

Thyroid hormones (THs) play a crucial role in development. At present, experimental studies on the cellular and molecular mechanisms underlying TH-dependent development have mainly concentrated on the later stages of development, while the role of THs during early development is less clear. The objective of this study is to further elucidate the role of THs in vertebrate embryonic development using zebrafish embryos.

Researcher(s)

Promotor: Knapen Dries

Research team(s)

Veterinary physiology and biochemistry

Development of a method for testing of transgenic rational effects of genetically modified crops in food on the basis of the zebrafish model (TRANSGGO).
01/01/2014 - 30/04/2018

Abstract

This project represents a formal research agreement between UA and on the other hand the Federal Public Service. UA provides the Federal Public Service research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

Promotor: Knapen Dries
Co-promotor: Blust Ronny

Research team(s)

Veterinary physiology and biochemistry

Pathways to subfertility: elevated non-esterified fatty acid concentrations as the causative link between maternal metabolic disorders and reduced embryo quality.
01/01/2014 - 31/12/2017

Abstract

This research project concentrates on the effect of oocyte maturation, fertilization and embryo culture under high NEFA conditions on fertilization rate, DNA transcription and methylation patterns in Day 7 embryos.

Researcher(s)

Promotor: Leroy Jo
Co-promotor: Bols Peter
Co-promotor: Van Cruchten Steven

Research team(s)

Veterinary physiology and biochemistry

Maternal metabolic disorders and the consequences for the egg cell and embryo quality: the impact of increased Free fatty acid concentrations during egg cell maturation on the development and differentiation of the pre-implantation embryo.
01/01/2014 - 31/12/2015

Abstract

This project represents a formal research agreement between UA and on the other hand the Flemish Public Service. UA provides the Flemish Public Service research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

High non-esterified fatty acid concentrations during preimplantation embryo development: Consequences for female fertility and offspring's health.
01/10/2013 - 30/09/2015

Abstract

To further expand on this intriguing patho-phsyiological role of elevated NEFA in the problem of subfertility, it need to be addressed whether NEFA can induce epigenetic changes and whether this affects further embryo development and postnatal health. Therefore, this research proposal concentrates on the effect of oocyte maturation under elevated NEFA conditions on DNA methylation patterns in Day 7 embryos, on further pre-implantation in vivo development and on postnatal health and growth. A state of the art in vitro embryo culture and embryo transfer protocols will be used combined with advanced molecular techniques. The integration of data on Day 7 embryo physiology, DNA methylation, further development and postnatal health could provide key metabolic information on the role of elevated NEFA concentrations in reproductive failure.

Researcher(s)

Promotor: Leroy Jo
Co-promotor: Bols Peter

Research team(s)

Veterinary physiology and biochemistry

Can antioxidants improve fertility in highly productive dairy cattle?
01/07/2013 - 30/09/2015

Abstract

This project represents a formal research agreement between UA and on the other hand the Province of Antwerp. UA provides the Province of Antwerp research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

Development of an alternative testing strategy for the fish early life-stage test for predicting chronic toxicity.
01/03/2013 - 29/02/2016

Abstract

The main objective of this project is to use a mechanistic framework to develop and propose a high-throughput tiered-testing strategy for screening and prioritizing chemicals for FELS testing (OECD 210). The main objective can be divided into four parts: 1) Establish a database of toxicologically relevant FELS-specific AOPs, identify molecular initiating events and subsequent intermediate responses resulting into the apical outcome of interest 2) Propose Tier 1 in vitro screening toolbox to test for AOP-specific events and responses predictive for FELS chronic toxicity. 3) Propose Tier 2 whole-organism ZFET assays to test for AOP-specific events and responses predictive for FELS chronic toxicity and assess the potential of a ZFET molecular screening tool to predict cellular, organ and/or organism responses giving rise to FELS chronic toxicity. 4) Offering a proposal for implementation of a tiered-testing strategy in EU regulation. Assessment of usefulness and applicability of tiered testing strategy for global scientific and regulatory community.

Researcher(s)

Promotor: Knapen Dries
Co-promotor: Blust Ronny

Research team(s)

Veterinary physiology and biochemistry

BOF: 1 year fellowship.
01/01/2013 - 31/12/2013

Abstract

This project represents a research contract awarded by the University of Antwerp. The supervisor provides the Antwerp University research mentioned in the title of the project under the conditions stipulated by the university.

Researcher(s)

Promotor: Leroy Jo
Co-promotor: Hermans Nina
Fellow: De Bie Jessie

Research team(s)

Veterinary physiology and biochemistry

The direct effects of elevated serum free fatty acid concentrations on the viability of ovarian follicles and the oocyte's developmental capacity.
01/10/2011 - 06/10/2013

Abstract

Previous research work revealed that metabolic changes, associated with the growing incidence of obese individuals and diabetics, might have harmful repercussions for the reproductive outcome. The consequences of hyperglycemia, due to obesity and diabetes, have already been investigated thoroughly in rats and mice. This research project rather involves another feature of these metabolic pathologies: the high free fatty acid concentrations in blood. The interest of the human assisted reproduction society in a bovine model to assess the influence of free fatty acids on the oocytes developmental competence, on the viability and function of the granulosa cells and the inquisitiveness to the underlying mechanism of these potential effects, imposes us to further research.

Researcher(s)

Promotor: Bols Peter
Co-promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

Fertility preservation and pregnancy outcome in cancer patients treated with radio and/or chemotherapy.
01/09/2011 - 31/08/2012

Abstract

This project represents a formal research agreement between UA and on the other hand a private institution. UA provides the private institution research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

Promotor: Bols Peter

Research team(s)

Veterinary physiology and biochemistry

Combined and interactive effects of climate change and chemical stress in Daphnia magna.
01/08/2011 - 31/12/2012

Abstract

This project represents a formal research agreement between UA and on the other hand UGent. UA provides UGent research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

Promotor: Knapen Dries

Research team(s)

Veterinary physiology and biochemistry

Advancing the zebrafish embryo as a model system in epigenomics – a study on the importance of DNA methylation dynamics in teratogenicity
01/07/2011 - 31/12/2015

Abstract

Teratogenic chemicals are prioritized under the EU REACH legislation. Within this framework there is a need for routine testing tools to investigate the mechanistic basis of teratogenicity and to determine whether a substance has teratogenic potential. It has become clear that not only genetic factors (DNA nucleotide sequence) but also epigenetic factors such as DNA methylation, regulating gene behaviour, play a crucial role in teratogenicity. During vertebrate early embryonic development a vital epigenetic reprogramming event takes place (the sequential demethylation and de novo remethylation of the embryonic genome). The extent to which disturbance of methylation mechanisms during embryonic stages is involved in the development of teratogenic effects is still unclear and will be the subject of this study. This project will use the zebrafish embryo, which is already an established test system for the study of developmental effects, as an alternative to using mammals for the study of the role of epigenetic factors in teratogenicity. We aim to achieve two goals: first, we will determine to what extent DNA methylation changes are responsible for transcription changes involved in teratogenicity. Secondly, we will identify and confirm key genes and pathways involved in teratogenicity based on the integration of the molecular data with developmental parameters. Zebrafish embryos will be exposed to different concentrations of chemicals with known teratogenic mode of action during different stages of embryonic development, either during or after the de novo remethylation event. The embryos will be monitored individually until hatching. A large set of developmental parameters will be scored (e.g. heart rate and morphological malformations of body shape, somites and tail). Additionally, after pre and post de novo methylation exposure, RNA and DNA will be extracted which will be used in gene expression and DNA methylation microarray analyses. Based on these microarray data we will establish to what extent differential gene expression that is directly involved in teratogenicity is determined by differences in methylation status. Finally, we will statistically link these molecular data with the observed developmental effects. Key genes will thus be identified which are linked to specific developmental abnormalities. The selected key genes will be confirmed by both bisulfite sequencing (gene specific DNA methylation status) and real-time PCR (gene specific mRNA levels). Although a few previously published studies focused on general methylation patterning in zebrafish, we would be the first to study methylation dynamics in zebrafish embryos in detail on a genome wide scale, linking such data to classical gene expression data. Advancing the zebrafish embryo as an alternative test system for epigenomics research will pave the way for its application in several areas of research including the development of a classifier able to identify teratogenic compounds and the identification of targets for disease prevention or therapy.

Researcher(s)

Promotor: Knapen Dries

Research team(s)

Veterinary physiology and biochemistry

Ilama/alpaca immunisation and blood collection.
15/05/2011 - 14/05/2012

Abstract

This project represents a formal service agreement between UA and on the other hand VIB. UA provides VIB research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

Pollution tolerance of Daphnia magna populations: evolutionary potential and interaction with natural stressors from an ecotoxicological, genomic and ecological perspective.
01/04/2011 - 31/12/2012

Abstract

This project represents a formal research agreement between UA and on the other hand KULeuven. UA provides KULeuven research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

Promotor: Knapen Dries

Research team(s)

Veterinary physiology and biochemistry

The consequences of high free fatty acid concentrations in the follicular microenvironment on follicular growth and on metabolic, genetic and epigenetic quality parameters of the oocyte and pre-implantation embryo.
07/03/2011 - 05/03/2013

Abstract

The project focuses on the long term culture of pre-antral follicles opens a whole new approach and frame work making it possible to study the effects of maternal nutrition and metabolism on the pre-ovulatory follicles and growing and maturing oocytes.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

The consequences of high free fatty acid concentrations in the follicular microenvironment on folliculogenesis and on metabolic, genetic and epigenetic quality parameters of the oocyte and pre-implantation embryo.
01/01/2011 - 31/12/2013

Abstract

A disturbed maternal metabolism may induce disappointing fertility and may jeopardize the offspring's health. Only recently, the importance of the early developmental stages in life has gained scientific attention in subfertility research. This project focuses on the metabolic and (epi)genetic consequences of long-term elevated non-esterified fatty acid (NEFA) serum concentrations in the dam on ovarian physiology, oocyte and embryo quality. Elevated NEFA cocnetrations are a key factor in several metabolic disorders such as diabetes, negative energy balance and obesitas and is typical for lipolysis. Previous research showed already that these elevated NEFA concentrations are reflected in the follicular fluid of the dominant follicle and that they cause a reduction in the oocyte's developmental competence. To mimic the in vivo situation even better and to substantiate the causative role of elevated NEFA in the pathogenesis of subfertility, a long term exposure model during the process of follicular growth should be developed. Therefore murine secondary follicles will be isolated and will be cultured for 12 days. These growing and maturing follicles will be exposed to elevated NEFA conditions and the effects on folliculogenesis, oocyte developmental competence and embryo quality will be studied in detail.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

Francqui Chair 2010-2011 Prof. Poul Hyttel.
01/10/2010 - 30/09/2011

Abstract

Proposed by the University, the Francqui Foundation each year awards two Francqui Chairs at the UAntwerp. These are intended to enable the invitation of a professor from another Belgian University or from abroad for a series of ten lessons. The Francqui Foundation pays the fee for these ten lessons directly to the holder of a Francqui Chair.

Researcher(s)

Promotor: Bols Peter
Co-promotor: Van Ginneken Chris

Research team(s)

Veterinary physiology and biochemistry

The consequences of high free fatty acid concentrations in the micro-environment of the oocyte and zygote on metabolic, genetic and epigenetic quality parameters of the pre-implantation embryo.
01/07/2010 - 31/12/2014

Abstract

A disturbed maternal metabolism may induce disappointing fertility and may jeopardize the offspring's health. Only recently, the importance of the early developmental stages in life has gained scientific attention in the study of the pathogenesis of subfertility. This project focuses on the metabolic and (epi)genetic consequences of long-term elevated non-esterified fatty acid serum concentrations in the dam on folliculogenesis, oocyte developmental competence and embryo quality.

Researcher(s)

Promotor: Leroy Jo
Co-promotor: Bols Peter
Co-promotor: Knapen Dries

Research team(s)

Veterinary physiology and biochemistry

Scientific research on food safety, health and animal welfare (EMBRYOSCREEN).
01/04/2010 - 28/02/2015

Abstract

This project represents a formal research agreement between UA and on the other hand the Federal Public Service. UA provides the Federal Public Service research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

Promotor: Bols Peter
Co-promotor: Knapen Dries
Co-promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

Fatty acids in dairy cattle in relation to human and animal health.
01/01/2010 - 31/12/2014

Abstract

This is a fundamental research project financed by the Research Foundation - Flanders (FWO). The project was subsidized after selection by the FWO-expert panel.

Researcher(s)

Promotor: Bols Peter
Co-promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

The effect of dietary poly-unsaturated fatty acids on the composition of follicular fluid and the consequences for oocyte and embryo quality. A combined bovine in vivo and in vitro model.
01/01/2010 - 31/12/2011

Abstract

Making the fatty acid composition of the human diet more poly-unsaturated is generally proposed as beneficial for health. This combined in vivo and in vitro bovine research model will elucidate the direct consequences of such a fatty acid shift on follicular fluid composition, on oocyte and embryo quality. Earlier work on dairy cows revealed several contradicting results, indicating the need for more in depth research.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

Improvement of Cuban dairy cattle genetics as an aid to increase milk production potential.
31/12/2009 - 11/12/2014

Abstract

While Cuba slowly recovers Gom an economical disaster in the nineties, there are numerous problems left asking for a sustainable solution, one of which is the severe structural shortage in milk production reflected in the absence of milk in the daily ration of many Cubans. A decline in support from former partners and the embargo lead to the loss of milk producing potential in the remaining cattle herds. The lack of operational reproduction techniques to be used as selection tools, severely decreased the absolute number of dairy cows. The current project aims to re-introduce dairy cattle genetics by creating a nucleus herd of cross bred bulls by applying modern cattle breedmg techniques to fertilize oocytes Gom the best local breed founder cows with (imported) dairy cattle sperm. The use of native breeds assures the cross bred products to be adapted to tropical conditions being resistant to relevant parasitic theads. Following this high-tech first phase of the project, the cross bred bulls themselves will propagate dairy cattle genetics in the population by simple, cheap and sustainable Artificial Insemination and natural mating. This way, relevant genetics can preferentially he made available to those farms which have access to the best cattle feeding conditions.

Researcher(s)

Promotor: Bols Peter

Research team(s)

Veterinary physiology and biochemistry

The influence of the metabolic status of the dam at conception on the glucose metabolism of the newborn calf.
04/12/2009 - 31/10/2011

Abstract

This project represents a formal research agreement between UA and on the other hand the Province of Antwerp. UA provides the Province of Antwerp research results mentioned in the title of the project under the conditions as stipulated in this contract.

Researcher(s)

Promotor: Leroy Jo
Co-promotor: Bols Peter

Research team(s)

Veterinary physiology and biochemistry

The direct effects of elevated serum free fatty acids concentrations on the viability of ovarian follicles and the oocyte's developmental capacity: an animal model.
01/10/2009 - 09/11/2011

Abstract

Previous research work revealed that metabolic changes, associated with the growing incidence of obese individuals and diabetics, might have harmful repercussions for the reproductive outcome. The consequences of hyperglycemia, due to obesity and diabetes, have already been investigated thoroughly in rats and mice. This research project rather involves another feature of these metabolic pathologies: the high free fatty acid concentrations in blood. The interest of the human assisted reproduction society in a bovine model to assess the influence of free fatty acids on the oocytes developmental competence, on the viability and function of the granulosa cells and the inquisitiveness to the underlying mechanism of these potential effects, imposes us to further research.

Researcher(s)

Promotor: Bols Peter
Co-promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

The direct consequences of elevated free fatty acids concentrations in the blood on the viability of ovarian follicles and the oocyte's developmental capacity: an animal model.
01/10/2008 - 30/09/2009

Abstract

Previous research work revealed that metabolic changes, associated with the growing incidence of obese individuals and diabetics, might have harmful repercussions for the reproductive outcome. The consequences of hyperglycemia, due to obesity and diabetes, have already been investigated thoroughly in rats and mice. This research project rather involves another feature of these metabolic pathologies: the high free fatty acid concentrations in blood. The interest of the human assisted reproduction society in a bovine model to assess the influence of free fatty acids on the oocytes developmental competence, on the viability and function of the granulosa cells and the inquisitiveness to the underlying mechanism of these potential effects, imposes us to further research.

Researcher(s)

Promotor: Bols Peter

Research team(s)

Veterinary physiology and biochemistry

Hyperlipidemia and the consequences on oocyte and embryo quality. A combined bovine in vivo and in vitro model for human infertility research.
01/01/2008 - 31/12/2009

Abstract

Hypercholesterolemia is a human disorder and it has been associated with an unhealthy eat pattern. This combined in vivo and in vitro bovine model will elucidate the effect of nutritionally induced hypercholesterolemia on oocyte and embryo quality. In earlier work we showed that the metabolic changes in dairy cows early post partum and their consequences on oocyte and follicle quality is a valuable model for research in human infertility.

Researcher(s)

Promotor: Leroy Jo

Research team(s)

Veterinary physiology and biochemistry

A study on the influence of Follicle Stimulating Hormone (FSH) and Insulin-like Growth Factor-I (IGF-I) on the follicular dynamics of pre-antral bovine ovarian follicles by means of transvaginal ultrasound-guided ovarian biopsies.
01/01/2004 - 31/12/2006

Abstract

The proposed study will investigate the impact of local treatment with FSH, IGF-I or a combination of both, on follicular dynamics and oocyte quality of bovine, pre-antral follicles in living donors. To do so, a new technique will be developed and validated, to take ovarian biopsies at regular time intervals during treatment, by means of repeated, ultrasound-guided, transvaginal puncture of the ovaries. This procedure, combined with histological examinations and in vitro culture techniques, will help us to investigate on which follicular stage FSH and IGF-I exert their stimulatory effect. The data collected will offer us new insights on the application of hormonal ovarian stimulation and lead to a better understanding of the primordial to primary stage follicle transition.

Researcher(s)

Promotor: Bols Peter

Research team(s)

Veterinary physiology and biochemistry

01/01/2003 - 31/12/2004

Abstract

Researcher(s)

Promotor: Bols Peter

Research team(s)

Veterinary physiology and biochemistry

01/05/2002 - 30/09/2004

Abstract

Researcher(s)

Promotor: Bols Peter

Research team(s)

Veterinary physiology and biochemistry

Maternale obesitas en "fetal programming": de gevolgen voor de voortplantingsfysiologie van de nakomelingen. 01/10/2018 - 30/09/2022

Abstract

Researcher(s)

Research team(s)

Can antioxidants ameliorate the development and quality of embryos under maternal metabolic stress conditions? 01/04/2018 - 31/03/2019

Abstract

Researcher(s)

Research team(s)

The first embryo-dependent and -independent programming of endometrial function in early pregnancy: a bovine model. 01/03/2018 - 31/08/2018

Abstract

Researcher(s)

Research team(s)

Development of strategic preconception care to improve maternal fertility and protect offspring's health in obese individuals: steps towards evidence based clinical advice. 01/01/2018 - 31/12/2021

Abstract

Researcher(s)

Research team(s)

Comparison of nano-injection and aquatic exposure in fish embryo tests: can differences in toxicity be explained by accumulation kinetics? 01/04/2017 - 31/03/2018

Abstract

Researcher(s)

Research team(s)

Mitochondrial and endoplasmic reticulum stress in oocytes and embryos as treatment targets for infertile patients with metabolic diseases. 01/04/2017 - 31/03/2018

Abstract

Researcher(s)

Research team(s)

reproduction as the cornerstone for sustainability in the dairy business: feeding for optimal fertility 01/01/2017 - 31/12/2017

Abstract

Researcher(s)

Research team(s)

Mother-to-embryo impact of maternal metabolic diseases: nutrient sensing pathways drive developmental and differentiation programs in the bovine embryo. 01/01/2017 - 31/08/2017

Abstract

Research team(s)

Mitochondria take centre stage: pathways to reduced oocyte quality and opportunities for curative strategies under maternal metabolic stress conditions. 01/10/2016 - 30/09/2019

Abstract

Researcher(s)

Research team(s)

Maternal obesity and uterine programming: the consequences for the offspring's reproductive physiology. 01/10/2016 - 31/12/2017

Abstract

Researcher(s)

Research team(s)

Development of an alternative testing strategy for the fish early-life stage test for predicting chronic toxicity : assay validation. 01/03/2016 - 28/02/2018

Abstract

Researcher(s)

Research team(s)

An alternative testing strategy for the fish early life-stage test using the adverse outcome pathway (AOP) framework. 01/01/2016 - 31/12/2016

Abstract

Researcher(s)

Research team(s)

The impact of endocrine disruption on vertebrate embryonic and larval development. 01/10/2015 - 30/09/2019

Abstract

Researcher(s)

Research team(s)

High non-esterified fatty acid concentrations during preimplantation embryo development: Consequences for female fertility and offspring's health. 01/10/2015 - 11/11/2017

Abstract

Researcher(s)

Research team(s)

Maternal metabolic disorders and early embryonic loss: pathways to bridge the gap between embryo quality and endometrial receptivity. 01/10/2015 - 31/08/2017

Abstract

Researcher(s)

Research team(s)

A functional study of the mechanisms underlying sex differentiation in zebrafish 01/02/2015 - 31/12/2015

Abstract

Researcher(s)

Research team(s)

Development of a zebrafish embryo test for environmental risk assessment of pharmaceuticals with estrogenic properties. 01/01/2015 - 31/12/2018

Abstract

Researcher(s)

Research team(s)

Maternal obesity and uterine programming: the consequences for the offspring's reproductive physiology. 01/10/2014 - 08/05/2015

Abstract

Researcher(s)

Research team(s)

Zebrafish embryos to elucidate the role of thyroid hormones in vertebrate embryonic development. 01/02/2014 - 31/12/2014

Abstract

Researcher(s)

Research team(s)

Development of a method for testing of transgenic rational effects of genetically modified crops in food on the basis of the zebrafish model (TRANSGGO). 01/01/2014 - 30/04/2018

Abstract

Researcher(s)

Research team(s)

Maternale obesitas en "fetal programming": de gevolgen voor de voortplantingsfysiologie van de nakomelingen.
01/10/2018 - 30/09/2022

Can antioxidants ameliorate the development and quality of embryos under maternal metabolic stress conditions?
01/04/2018 - 31/03/2019

The first embryo-dependent and -independent programming of endometrial function in early pregnancy: a bovine model.
01/03/2018 - 31/08/2018

Development of strategic preconception care to improve maternal fertility and protect offspring's health in obese individuals: steps towards evidence based clinical advice.
01/01/2018 - 31/12/2021

Comparison of nano-injection and aquatic exposure in fish embryo tests: can differences in toxicity be explained by accumulation kinetics?
01/04/2017 - 31/03/2018

Mitochondrial and endoplasmic reticulum stress in oocytes and embryos as treatment targets for infertile patients with metabolic diseases.
01/04/2017 - 31/03/2018

reproduction as the cornerstone for sustainability in the dairy business: feeding for optimal fertility
01/01/2017 - 31/12/2017

Mother-to-embryo impact of maternal metabolic diseases: nutrient sensing pathways drive developmental and differentiation programs in the bovine embryo.
01/01/2017 - 31/08/2017

Mitochondria take centre stage: pathways to reduced oocyte quality and opportunities for curative strategies under maternal metabolic stress conditions.
01/10/2016 - 30/09/2019

Maternal obesity and uterine programming: the consequences for the offspring's reproductive physiology.
01/10/2016 - 31/12/2017

Development of an alternative testing strategy for the fish early-life stage test for predicting chronic toxicity : assay validation.
01/03/2016 - 28/02/2018

An alternative testing strategy for the fish early life-stage test using the adverse outcome pathway (AOP) framework.
01/01/2016 - 31/12/2016

The impact of endocrine disruption on vertebrate embryonic and larval development.
01/10/2015 - 30/09/2019

High non-esterified fatty acid concentrations during preimplantation embryo development: Consequences for female fertility and offspring's health.
01/10/2015 - 11/11/2017

Maternal metabolic disorders and early embryonic loss: pathways to bridge the gap between embryo quality and endometrial receptivity.
01/10/2015 - 31/08/2017

A functional study of the mechanisms underlying sex differentiation in zebrafish
01/02/2015 - 31/12/2015

Development of a zebrafish embryo test for environmental risk assessment of pharmaceuticals with estrogenic properties.
01/01/2015 - 31/12/2018

Maternal obesity and uterine programming: the consequences for the offspring's reproductive physiology.
01/10/2014 - 08/05/2015

Zebrafish embryos to elucidate the role of thyroid hormones in vertebrate embryonic development.
01/02/2014 - 31/12/2014

Development of a method for testing of transgenic rational effects of genetically modified crops in food on the basis of the zebrafish model (TRANSGGO).
01/01/2014 - 30/04/2018

Pathways to subfertility: elevated non-esterified fatty acid concentrations as the causative link between maternal metabolic disorders and reduced embryo quality.
01/01/2014 - 31/12/2017

Maternal metabolic disorders and the consequences for the egg cell and embryo quality: the impact of increased Free fatty acid concentrations during egg cell maturation on the development and differentiation of the pre-implantation embryo.
01/01/2014 - 31/12/2015

High non-esterified fatty acid concentrations during preimplantation embryo development: Consequences for female fertility and offspring's health.
01/10/2013 - 30/09/2015

Can antioxidants improve fertility in highly productive dairy cattle?
01/07/2013 - 30/09/2015

Development of an alternative testing strategy for the fish early life-stage test for predicting chronic toxicity.
01/03/2013 - 29/02/2016

BOF: 1 year fellowship.
01/01/2013 - 31/12/2013

The direct effects of elevated serum free fatty acid concentrations on the viability of ovarian follicles and the oocyte's developmental capacity.
01/10/2011 - 06/10/2013

Fertility preservation and pregnancy outcome in cancer patients treated with radio and/or chemotherapy.
01/09/2011 - 31/08/2012

Combined and interactive effects of climate change and chemical stress in Daphnia magna.
01/08/2011 - 31/12/2012

Advancing the zebrafish embryo as a model system in epigenomics – a study on the importance of DNA methylation dynamics in teratogenicity
01/07/2011 - 31/12/2015

Ilama/alpaca immunisation and blood collection.
15/05/2011 - 14/05/2012

Pollution tolerance of Daphnia magna populations: evolutionary potential and interaction with natural stressors from an ecotoxicological, genomic and ecological perspective.
01/04/2011 - 31/12/2012

The consequences of high free fatty acid concentrations in the follicular microenvironment on follicular growth and on metabolic, genetic and epigenetic quality parameters of the oocyte and pre-implantation embryo.
07/03/2011 - 05/03/2013

The consequences of high free fatty acid concentrations in the follicular microenvironment on folliculogenesis and on metabolic, genetic and epigenetic quality parameters of the oocyte and pre-implantation embryo.
01/01/2011 - 31/12/2013

Francqui Chair 2010-2011 Prof. Poul Hyttel.
01/10/2010 - 30/09/2011

The consequences of high free fatty acid concentrations in the micro-environment of the oocyte and zygote on metabolic, genetic and epigenetic quality parameters of the pre-implantation embryo.
01/07/2010 - 31/12/2014

Scientific research on food safety, health and animal welfare (EMBRYOSCREEN).
01/04/2010 - 28/02/2015

Fatty acids in dairy cattle in relation to human and animal health.
01/01/2010 - 31/12/2014