Dirk Ysebaert emeritus with assignment

Research Dirk Ysebaert

Research team

Neural Stem Cells: molecular and physiological control of in vivo migration and differentiation. 01/01/2011 - 31/12/2014

Abstract

This proposed multidisciplinary research consortium, consisting of 6 different laboratories from the University of Antwerp, aims to understand the cellular and/or functional interactions of NSC implants in healthy and injured neural tissue (cuprizone-mediated demyelinisation mouse model). With this research project, which focuses on the in vivo molecular and physiological control of NSC, we aim to contribute to the in vivo study and modulation of NSC migration, survival, differentiation and functional integration.

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    Control of glioblastoma by modulation of the brain's innate immune responses. 01/01/2011 - 31/12/2014

    Abstract

    We first aim to identify and functionally describe immune-suppressive proteins and/or signalling molecules on glioma cells, which lead to inhibition of microglia. Then, we will develop strategies to modify microglia in order to prevent inhibition by glioma cells in vitro and in vivo. We specifically aim to genetically engineer microglia with 'short interfering RNAs' against receptors or signalling molecules involved in immune inhibition by GL261 cells in order to improve their cellular therapeutic potency.

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      Characterisation of innate immune responses in the central nervous system: modulation towards immunological acceptance of allogeneic cellular grafts. 01/01/2011 - 31/12/2014

      Abstract

      In this project, we aim to further elucidate the mechanisms leading to immune-mediated rejection of allografts in the CNS. For this, we will non-invasively (by in vivo bioluminescence imaging) identify the exact timing and degree of microglia immune-reactivity in relation to immune-mediated rejection of different allogeneic adult-, embryo- and placenta-derived cell populations.

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        Influence of progenitor cells to liver regeneration after major resection in pathological liver conditions. 01/01/2011 - 31/12/2012

        Abstract

        This project represents a research agreement between the UA and on the onther hand IWT. UA provides IWT research results mentioned in the title of the project under the conditions as stipulated in this contract.

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        Detection, isolation and culture of liver progenitor cells after liver resection in a model of chronic liver damage in the rat 01/07/2009 - 31/12/2013

        Abstract

        The study will investigate which progenitor cells are involved in liver regeneration, and if there is contributionof CD133 stem cells to the liver after resection in a predamaged liver by chemo & non-alcholic steatohepatitis in mice. Next, recruitment and eventual improvement of liver regeneration after infusion of CD133 cells will be studied. This study will give more insight in liver regeneration in pathologcial conditions which can be applied in the treatment of patients.

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        Clinical and preclinical research of the effect of cellular mediators on the modulation of pathogenic responses in multiple sclerosis. 01/01/2009 - 31/12/2012

        Abstract

        In this project, we want to further investigate and exploit the capacity of DC and Treg to correct or modulate pathogenic responses in MS patients. Current research will provide the foundation for the eventual development of a cellular vaccine for the treatment of MS. Depending on the results of this study it can be envisaged to treat patients suffering from MS with tolerogenic DC and/or immunosuppressive Treg in order to eliminate or inactivate autoreactive T cells.

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          Influence of progenitor cells to liver regeneration after major resection in pathological liver conditions. 01/01/2009 - 31/12/2010

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          Cell biological and experimental study of the mechanisms and modulators underlying the protective effect of erythropoietin. 01/07/2007 - 31/12/2011

          Abstract

          Erythropoietin (EPO) has recently been shown to protect the kidney both functionally and morphologically against acute renal failure induced by ischemia/reperfusion injury, as seen for example after kidney transplantation. The mechanisms underlying this renoprotective effect are not known. This project aims to elucidate the mechanisms of the EPO-induced renoprotection both in vivo (ischemic rat model) and in vitro (human renal tubular cells culture), this in order to justify further clinical studies.

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          Transplantation of embryonic stem cell-derived neural stem cells after spinal cord injury and traumatic brain injury. 01/01/2007 - 31/12/2010

          Abstract

          This project will investigate whether transplantation of defined embryonic stem cell-derived neural stem cells (ES-NSC), genetically modified to secrete neurotrophic factors, can support or improve recovery after TBI and SCI. An improved recovery can be due to: A) a decreased secondary neural loss due to secretion of neurotrophic factors, and/or B) an enhanced neural recovery due to functional integration of transplanted ES-NSC and/or recruited endogenous NSC.

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            Expression of genes involved in inflammation and fibrosis in protocolbiopsies of renal allografts. A study of the pathophysiologic mechanisms of chronic allograft nephropathy. 01/01/2004 - 31/12/2007

            Abstract

            In a prospective, randomized study in de novo kidney transplant recipients, we will compare the influence of a combination therapy with prednisolone, mycophenolate mofetil, and cyclosporine with a combination therapy with prednisolone, mycophenolate mofetil, and rapamycine, on the expression of genes, associated with inflammation and fibrosis in protocol biopsies of the graft, six months after transplantation. Glomeruli, tubulo-epithelial cells and interstitial cells will be isolated by means of the laser capture microscope. After RNA-extraction, we will perform semiquantitative RT amplification with Taqman PCR for the genes of interest: i.e. IL 10, IL 12, TNF alpha, B7-1, B7-RP1, CD40, CXCR3, CCR5, perforine, granzyme, angiotensine II, TGF-ß1, PAI-1, TIMP-1, and endotheline.

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            01/10/2001 - 30/09/2004

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            Influence of immunosuppressiva on the regeneration of the transplanted kidney after ischemia - reperfusion. 30/09/1995 - 31/12/1996

            Abstract

            Renal transplantation can be regarded as a model of kidney ischemia reperfusion, with a neutrophils cellular infiltrate, superimposed by an eventual allograft rejection infiltrate. Acute renal failure is still a frequent complication in the immediate post-transplant period, very often as a result from ischemic injury, cyclosporine toxicity, acute rejection or a combination of these factors. The regeneration of the transplanted kidney after ischemia - reperfusion, seems to be delayed. As far as immunosuppression plays a rule on this recovery, is the subject of investigation on a rat model of renal transplantation.

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