Research Jan Dries

Abstract

The project is situated in the domain of environmental biotechnology. For over a century, the activated sludge process has been the most commonly used method of biological wastewater treatment. Despite a high efficiency in removing pollutants, its sustainability is now questioned. Aerobic granular sludge (AGS) is a recent innovation that allows for efficient wastewater treatment, while recovering resources and minimizing land footprint and energy consumption. However, an important issue that has poorly been recognized is the production of nitrous oxide (N2O) during nitrogen removal in AGS. N2O is a potent greenhouse gases with a global warming potential 300 times higher than carbon dioxide. The aim of the project is to develop a model to optimize N2O emission mitigation in AGS. The model will be extensively calibrated, validated and ultimately confirmed based on experimental data obtained from laboratory-scale AGS reactors performing integrated nitrogen and phosphorus removal. The project combines the complementary expertise of researchers of the Gda?sk University of Technology (Poland) and the University of Antwerp (Belgium). The project will identify key operational and microbial mechanisms of N2O production, and will result in experimentally confirmed model-based mitigation strategies to minimize and avoid N2O emission. The project results will thus significantly contribute to the sustainability of the innovative AGS process for wastewater treatment

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

To bring the world closer to "clean water for all", this project focuses on the aerobic granular sludge (AGS) technology as a more sustainable alternative for the current activated sludge systems (less energy and footprint and higher resource recovery potential). Especially industrial wastewaters, rich in, e.g., lipids and proteins, hold much potential but are underexploited in AGS applications due to their complex and variable composition. Hydrolysis is indeed most often required and rate limiting. Moreover, if the influent required hydrolysis capacity is not met by the AGS' hydrolysis capacity, then the granulation is impaired. With as first objective to a enable robust AGS reactor operation for industrial wastewaters, this project will (i) develop an extensive set of monitoring tools, focusing on hydrolysis capacity, storage and structural polymers and other activity measurements, (ii) develop a synthetic granule community to enable clear conclusions when the impact of the influent complexity is studied and (iii) develop a control strategy to boost and tune the AGS' hydrolysis capacity. In general, granulation is enabled by structural polymers (stEPS), induced by feast-famine conditions, but also the slow growing organisms that store internal polymers are said to contribute. Both the stEPS and the storage polymers have industrial value when recovered. The project, therefore, also investigates waste granule pretreatment techniques to maximize (storage) polymer recovery.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The aim of the project is to demonstrate that ozonation (O3) in combination with Granular Activated Carbon (GAC) can be used at WWTPs as an innovative, effective and cost-efficient post-treatment technique to improve surface water quality in the Netherlands and Flanders. Ozonization is the efficient technique for the removal of a wide range of organic micropollutants (OMPs) but has the disadvantage that it can lead to the release of harmful by-products such as bromate and transformation products. A higher ozone dosage, which is required for far-reaching removal of OMPs, also leads to a greater risk of the formation of by-products in this process. With GAC filtration, these by-products are not formed, however, frequent regeneration of the activated carbon is necessary when using GAC, leading to high costs and a large carbon footprint. This project therefore investigates how these two technologies can be best integrated so that the highest possible OMP removal can be achieved with minimal by-product formation, minimal GAC regeneration and optimal energy and resource consumption.

Researcher(s)

• Promoter: Dries Jan
• Co-promoter: De Boeck Gudrun

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The aerobic granular sludge process (AGS) is a revolutionary development in the field of biological wastewater treatment. The formation of dense and compact granules leads to a significant intensification of the process, with a lower footprint, decreasing energy consumption and reduced sludge formation. Currently, however, all granular sludge reactors are of the batch type, with discontinuous feeding. This greatly limits the market potential of the technology. This project, a collaboration between research groups from Flanders and Vietnam, aims to develop a continuously fed granular sludge process. The knowledge of the AGS process, at UAntwerpen, is translated to the continuous process "ICEAS" (intermittent cycle extended aeration system) in which the Vietnamese German University has built a strong expertise. Furthermore, at UAntwerp, we are investigating the dynamics of formation and removal of the strong greenhouse gas nitrous oxide (N2O) during simultaneous nitrogen and phosphorus removal by granular sludge systems, in order to elaborate a N2O mitigation strategy. At the same time, the granular ICEAS concept will be optimized, in Vietnam, for the treatment of wastewater from the seafood processing industry, an important economic activity in Vietnam with a considerable ecological impact. The expected outcome of the project is an innovative and sustainable continuously fed granular sludge process, achieved through the cooperation of both partners.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

More efficient, compact, and less energy consuming technologies for wastewater treatment are urgently needed to meet the ever stricter discharge norms and to overcome the overall shortage of water, which necessitates water reuse. By combining both membrane bioreactor (MBR) and aerobic granular sludge (AGS) technologies, the main disadvantages of the current conventional activated sludge system (CAS) (e.g. being energy and space intensive and less prone for a compact C/N/P removal or recovery) can be overcome. While the granules can potentially alleviate membrane fouling, the feast-famine conditions and/or other alledged granulation parameters are not straightforwardly transferred to a continuous system with complete sludge retention such as in an MBR. This research aims to achieve and maintain stable granulation of aerobic granular sludge and integrate it with membrane bioreactors in continuous-flow reactors (CFRs). Our central research hypothesis is that the main granulation mechanism, i.e. microbial selection, can be applied to continuous-flow systems. A stepwise approach from sequencing batch to continuous and from continuous to MBR operation, with simple and complex substrates will lead to a final validation with real industrial wastewater. Thorough microbial analysis will gain the required insights to boost the AGS-MBR process to be widely applied in full scale wastewater treatment plants.

Researcher(s)

• Promoter: Dries Jan
• Fellow: Li Zehao

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

PFAS, poly- or perfluoroalkyl substances, includes thousands of different man-made fluorinated compounds with all kinds of applications, in fire-fighting foams, textiles, building materials, coatings, etc. However, PFAS are also "forever chemicals": persistent, mobile and barely biodegradable. PFAS occur in groundwater and surface water, and a major source of pollution is industrial wastewater. The current technology for removing PFAS from wastewater is activated carbon filtration, where PFAS bind to activated carbon via sorption. In this process no degradation occurs, and the long term efficiency is strongly dependent on the properties of PFAS (e.g. chain length, functional groups...). In the project we want to evaluate and optimize (combinations of) different existing techniques for the removal of PFAS from industrial wastewater, including the cost/benefit analysis and scalability. In addition, we want to gain insight in the behavior of PFAS in industrial (biological) wastewater treatment plants, more specifically how the operation of the biology influences PFAS removal by binding to the activated sludge. The proposed technologies include (electro-) coagulation techniques, various (advanced) chemical oxidation techniques (AOP), electrochemical oxidation (EO), and membrane filtration. We investigate not only the applicability to the effluent, but also to the concentrate stream after membrane filtration in a water reuse scenario.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

Enviromics is a multidisciplinary consortium of UAntwerpen researchers across the board of environmental sciences and technologies. Through impactful fundamental advances and interdisciplinary approaches across biology, (bio)chemistry and (bio)engineering, the consortium offers bio based solutions to ecosystem challenges by a strong interaction between three pillars (i) Environmental applications and nature based solutions, (ii) Sensing and analysis of chemicals and environments and (iii) Microbial technology and biomaterials, supported by sustainable product development and technology assessment. Through a renewed and tighter focus the ENVIROMICS consortium now signs for a leaner and more dynamic shape. Through intensified collaborations with different stakeholders, both national and international, the leverage for creating enhanced business and societal impact is reinforced. The consortium is strongly managed by a team of two highly profiled researchers partnered by an IOF manager and a project manager with clearly defined tasks and in close contact with the consortium members and the central Valorisation Unit of the university. The consortium has a strong and growing IP position, mainly on environmental/electrochemical sensing and microbial probiotics, two key points of the research and applications program. One spinoff was created in 2017 and two more will be setup in the coming three years. The direct interaction with product developers ensures delivering high TRL products. Next to a growing portfolio of industrial contracts, we create tangible societal impact, when relevant including citizen science approaches. Through the stronger leverage created by the new structure and partnerships we will develop both intertwined branches significantly.

Researcher(s)

• Promoter: Blust Ronny
• Co-promoter: De Wael Karolien
• Co-promoter: Dries Jan
• Co-promoter: Du Bois Els
• Co-promoter: Lebeer Sarah
• Co-promoter: Meire Patrick
• Co-promoter: Meysman Filip
• Co-promoter: Samson Roeland
• Co-promoter: Vandermoere Frederic
• Co-promoter: Vlaeminck Siegfried
• Fellow: Dardenne Freddy

Research team(s)

• Ecosphere

Project type(s)

• Research Project

Abstract

PROMIPOL brings together an interdisciplinary team to provide a revolutionary new route for the production of polymers. We will do this by growing bacteria on building blocks derived from CO2 and CO such as ethanol and methanol. The bacteria are rich in protein (>60% of the dry weight) and whereas it is well known that certain proteins can be used e.g. to produce foils for food packaging there is today no knowledge on microbial protein as source of polymers for packaging or other applications. Moreover, depending on the growth conditions the bacteria can also produce polyhydroxyalkanoates (PHA) as a second polymer class. We will grow bacteria that are food grade, finetune ratios between proteins and PHA and then extract these two sources together or separately to maximize carbon yields. By processing these polymers with variable composition e.g. through extrusion and investigating their properties, we will for the first time be able to assess whether they can be used for food packaging or other applications. Based on the applications, potential markets will be investigated and the key research questions will be identified towards future joint projects.

Researcher(s)

• Promoter: Vlaeminck Siegfried
• Co-promoter: Dries Jan

Research team(s)

• Sustainable Energy, Air and Water Technology (DuEL)

Project type(s)

• Research Project

Abstract

The BioPOM project aims at the application of Enhanced Biological Phosphorus Removal (EBPR) from potato processing industrial wastewater. The EBPR process not only reduces the amount of chemicals needed for phosphorus removal, but also significantly lowers the salinity of the treated water, thus allowing reuse of the treated effluent for irrigation purposes.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The service platform BioGRAN is situated in the domain of environmental biotechnology, i.e. the biological treatment of industrial wastewater. BioGRAN aims at assisting companies, that treat their own wastewater, in the implementation of the revolutionary aerobic granular sludge technology, leading to significant economic and sustainability advantages. In a first phase, BioGRAN determines the feasibility of granulation with the specific wastewater from a client company. The translation of the key granulation parameters to practical implementation subsequently happens in collaboration with an external partner, the water consultant Cre@Aqua. In this phase, BioGRAN is involved in monitoring of the biological treatment plant during, and after, the implementation of the granular sludge technology.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The BioWAVE research group is part of the LED-water network: academics giving first line of advice for companies, in all water-related areas. BioWAVE is the point of contact for companies in the province of Antwerp.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

In this project we investigate the impact of the aerobic granular sludge (AGS) technology on the membrane filtration characteristics in a membrane bioreactor (MBR) treating industrial wastewater, on full scale.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

BR 2.0 - Optimization of industrial biological wastewater treatment. About one-third of the industrial biological wastewater treatment plants suffer from poor sludge quality, often due to excessive growth of filamentous bacteria. This results in problematic settling, poor sludge dewatering, difficult sludge/water separation and even sludge washout in the effluent. The project aims to apply an innovative strategy to improve the structure, and thus the settling properties, of industrial activated sludge. This is done by acting on the plant's process operation to stimulate favorable groups of bacteria in the sludge, leading to compact sludge flocs. This strategy thus acts on the cause of poor settling! This innovative strategy was applied on a lab scale to improve the quality of activated sludge originating from different companies from various industries. The lab tests were conducted with sludge (1) from the food sector without nutrient removal, (2) from the food sector with nutrient removal, and (3) from the tank cleaning sector with complex wastewaters. The strategy was also applied at full scale in an industrial activated sludge plant. Here the operational strategy was optimized to obtain good and stable sludge quality. In all cases, good sludge quality was obtained, manifested by good sludge sedimentation (SVI < 100 ml/g), good effluent quality, and compact sludge morphology.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The BioWAVE research group is part of the LED-water network: academics giving first line of advice for companies, in all water-related areas. BioWAVE is the point of contact for companies in the province of Antwerp.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

Diluted phenol-rich streams occur regularly in lignocellulose-based biorefineries. Today, phenols are often regarded as waste. Some microorganisms can convert phenols into valuable intracellular components by fermentation. This makes the troublesome waste stream a raw material and an economic opportunity. To efficiently concentrate these dilute phenolic streams by conversion to intracellular components, it is necessary to speed up the process. In practice this often happens by increasing the amount of microorganisms, the biocatalyst, and consequently creating high cell concentrations. There is no efficient economic process for integrated fermentation and recovery of intracellular products. Our hypothesis is that the design of a new reactor type, namely a reversible immobilized cell reactor (RIR), offers a possible solution. In this reactor successively adhesion of the cells on a suitable support, fermentation, and finally desorption, to recover the intracellular components occurs. As a case study, the production of microbial oil is investigated starting from the phenolic hydrolysate obtained during the thermochemical treatment of lignocellulose. The aim of this project is to design an economically feasible process for valorising this phenolic flow. The new process will contribute to obtaining a biomass based circular economy.

Researcher(s)

• Promoter: Cornet Iris
• Co-promoter: Dries Jan
• Co-promoter: Vlaeminck Siegfried
• Fellow: Broos Waut

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The main aim of this proof of concept project is the development of an dynamic algorithm which makes use of simple and reliable sensor signals in order to monitor the anaerobic storage reaction. The motivation of this monitoring tool is to achieve well settling activated sludge (SVI < 100 mL/g) for the biological treatment of industrial wastewater.

Researcher(s)

• Promoter: Dries Jan
• Co-promoter: Dobbeleers Thomas

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

Aerobic granular sludge (AGS) is a revolutionary biological wastewater treatment system, with significant advantages in comparison to conventional activated sludge. A very interesting feature of AGS is the potential for simultaneous biological nitrogen (N) and phosphorus (P) removal from wastewater. This reaction relies on the specific storage metabolism of denitrifying polyphosphate-accumulating organisms (dPAO). Biological N-removal is however also linked to the formation of the potent greenhouse gas nitrous oxide (N2O). Specifically in AGS, unbalanced denitrification may lead to accumulation of the intermediate N2O, due to the slow consumption of storage polymers by dPAO. The most intensively studied (d)PAO in AGS are Accumulibacter species, but these bacteria have a substrate range limited to the volatile fatty acids (VFA) acetate and propionate. Other putative dPAO using broader substrate ranges have been identified in (conventional) biological wastewater treatment systems, but their role in granulation and N2O formation/reduction dynamics remains unexplored. The main objectives of the current proposal are to investigate (1) the formation of AGS, and (2) the dynamics of P-removal coupled to denitrification and N2O formation/reduction in AGS, in well-defined enrichments of putative dPAO, by applying single and mixed carbon substrates (i.e. VFA, amino acids, glucose). The long-term enrichments are supported by (1) short-term batch experiments to investigate microbial activities, (2) microbial analyses (using quantitative PCR and 16S rRNA gene amplicon sequencing analysis) to monitor the taxonomic composition of the enriched microbial communities, and (3) analyses of gene expression of the key denitrification genes. The knowledge gained will (1) increase our insight in the complex reactions in granular systems, offering opportunities (2) to optimize nutrient cycling in AGS reactors, and (3) to define much-needed strategies to limit and mitigate N2O emissions from these innovative wastewater treatment plants

Researcher(s)

• Promoter: Dries Jan
• Fellow: Dockx Lennert

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The research group BioGEM acts as the contact point for companies located in the province of Anwerp, for all types of water-related issues. The project involves (1) free advice, (2) small scale tests, (3) formulation of theses, and (4) initiation of larger projects.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

This projected is situated in the field of biological wastewater treatment and more specifically deals with the activated sludge (AS) process. Filamentous bulking due to the overgrowth of filamentous organisms has been generally reported as one of the most significant downsides of the AS process. In order to provide solutions for (waste)water practitioners, it is crucial to determine the type of these filamentous organisms and link their dominance to external factors. In this project we aim to use next generation sequencing (16s rRNA amplicon sequencing) to analyze the microbial community and more specifically the presence of filamentous organisms of 30 industrial wastewater treatment plants, relevant for the region of Flanders. Subsequently we aim to link the obtained microbial data to external factors such as wastewater, biomass characteristics and the operational parameters of the treatment plant.

Researcher(s)

• Promoter: Dobbeleers Thomas
• Co-promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

Industrial wastewater may contain non-biodegradable organics (recalcitrant COD). In the project, we investigate smart combinations of chemical and biochemical technologies to treat such wastewaters. The project focuses on wastewater from recycling processes.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The project AEROGRAM aims at implementing an innovative wastetwater treatment technology in industry, consisting of the integration of the aerobic granular sludge technology (AGS) in a membrane bioreactor (MBR). The granular MBR (GMBR) thus combines the advantages of granular sludge (small footprint, dense sludge, simultaneous nutrient removal) and MBR (excellent effluent quality, water reuse).

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The research group BioGEM acts as the contact point for companies located in the province of Anwerp, for all types of water-related issues. The project involves (1) free advice, (2) small scale tests, (3) formulation of theses, and (4) initiation of larger projects.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project website

Project type(s)

• Research Project

Abstract

Aerobic granular sludge (AGS) represents a true revolution in the field of biological wastewater treatment with significant advantages in comparison to conventional activated sludge, such as low energy use, small footprint, process efficiency, recovery of biopolymers & phosphorus...). The granulation process relies on the specific metabolism of carbon-storing bacteria, such as polyphosphate accumulating organisms (PAO). The most intensively studied PAOs in AGS are Accumulibacter species, but these bacteria have a very narrow substrate range (i.c. the volatile fatty acids VFA acetate and propionate). Other abundant putative PAOs using broader substrate ranges have been identified in (conventional) biological wastewater treatment systems, but their role in granulation and biopolymer formation remains unexplored. The main objective of the current proposal is to investigate (1) the formation of AGS, and (2) the production of the valuable gel-forming alginate-like extracellular polysaccharides (ALE), in well-defined enrichments of putative PAOs, other than Accumulibacter, by applying carbon substrates other than the VFAs acetate and propionate. Advanced microbial analyses, i.e. quantitative PCR (qPCR) and 16S rRNA gene amplicon sequencing analysis, will be used to monitor taxonomic composition of the microbial community in the sludge during the enrichments. The results of the project will have a significant impact on the development of the aerobic granular sludge technology. The results will help define the application potential of the technology, by pointing out the type of wastewaters that can be treated. Increased knowledge of the key PAOs involved in granulation is also very useful in view of the optimization of the treatment process as it allows tuning the reactor operation in favor of the desired PAO.

Researcher(s)

• Promoter: Dries Jan
• Co-promoter: D'aes Jolien
• Fellow: Dockx Lennert

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The project involves the set-up of a fully automated labscale biological wastewater treatment unit and respirometer unit for the professional bachelor program in chemistry/biochemistry at the KdG University College.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The main aim of this innovation mandate is to develop a compact and sustainable granular sludge system allowing the treatment of a continuous flow of variable industrial wastewater. First, a dynamic process control strategy will be developed at lab-scale. Afterwards, this technology/strategy will be examined at pilot-scale, at the location of a potential industrial user. A successful project may lead to the commercialization of an energy-efficient, compact and flexible technology for the treatment of industrial wastewater

Researcher(s)

• Promoter: Dries Jan
• Fellow: Dobbeleers Thomas

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The project involves the set-up of a fully automated labscale biological wastewater treatment unit for the professional bachelor program in chemistry/biochemistry at the Karel de Grote (KdG) University College.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

Tank truck cleaning (TTC) operations generate a highly complex wastewater. Even after treatment, TTC wastewater may still exhibit a high ecotoxic impact. In this project, we investigate different combinations of chemical and biological oxidation methods to remove residual ecotoxicity from TTC wastewater.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

Aerobic granular sludge (AGS) is an innovative development in biological wastewater treatment. However, AGS systems are very complex, and to be able to further exploit the potential of AGS, more insight is needed into the different factors governing their formation, maintenance, and composition. The goal of this project is to gain more insight into the properties and composition of aerobic granules, and into the mechanism of their formation. To this end, we will explore the feasibility of obtaining aerobic granular biofilms with a pure microbial culture of Pseudomonas sp. CMR12a or with a mixed microbial culture, starting from a pure-culture inoculum.

Researcher(s)

• Promoter: D'aes Jolien
• Co-promoter: Dries Jan
• Co-promoter: Lebeer Sarah

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

Nitrous oxide (N2O) is an important greenhouse gas, having a 300-fold stronger effect than carbon dioxide. N2O emissions are associated with nitrogen-removal processes in biological wastewater treatment, and contribute significantly to the total carbon footprint of a treatment plant. Aerobic granular sludge (AGS) is an innovative biological treatment technology leading to significant energy savings in the water treatment process. Knowledge on N2O emissions from these innovative AGS systems is however missing. The objective of the project is therefore the quantification of N2O formation in well-defined AGS systems.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

Aerobic granular sludge (AGS) represents a revolutionary new biological treatment system based on the formation of very dense microbial aggregates. The current research proposal aims at developing an applicable dynamic control strategy for industrial AGS reactors, based on the signals of common, low-cost and robust sensors. A five step approach will be followed to reach this objective, starting from the development of a control strategy for "simple" well-defined AGS systems on laboratory scale to pilot-scale evaluation of an optimized control algorithm with real variable industrial wastewater.

Researcher(s)

• Promoter: Dries Jan
• Co-promoter: Copot Cosmin

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The TNAV project aims at promoting the integration online sensors in the operation of industrial wastewater treatment plants. The implementation of online sensors significantly reduces the energy demand of the installation, and the use of chemicals.

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

Mannosylerythritol lipids (MELs) are one of the most promising biosurfactants (BS) because of their many potential applications. Despite all the advantages, BS and MELs in particular, are merely fermented on a limited scale. The main reasons for this are: (1) a limitation in structural variation of naturally occurring BS and (2) the cost, which is often not competitive with that of the chemically synthesized surfactants. The high cost is to a large extent determined by the cost of the feedstock. MELs are currently produced from soybean oil, a valuable substrate that increases the load on fertile land use. A possible alternative to vegetable oil could be oil from oleaginous yeasts. For several of these yeasts it has been shown that they are able to produce oil from volatile fatty acids, a very inexpensive substrate which can be obtained through acidogenesis of wastewater, an abundant waste stream. The objective of this project can be comprised in the research components below, each responding to a limiting factor of MEL-production: - Cost of the substrate: The development and optimization of a multi-stage production system where MELs are produced from oil fermented by the oleaginous yeast Cryptococcus curvatus. For the production of oil by C. curvatus, volatile fatty acids (VFA) and / or glycerol will be used as feedstock. Finally, we designed a case study to obtain VFA from industrial wastewater. - Limited structural variation: In order to obtain a structural variety of MEL-derivatives, alternative feeding of fatty acids and derivatives, such as hydroxylated and bifunctional fatty acids, are fed instead of (vegetable) oil. Hereby we will identify what the most promising varieties are, and their production will be optimized. - Limitations in fermentation yield: Elucidation of the trigger effect of a hydrophobic carbon source in MEL production by Pseudozyma aphidis. To achieve this, the expression of MEL biosynthesis genes will be analyzed under various conditions, with the aid of RT-qPCR. This is also a support towards the other research components. For the production of MELs and oil, fermentation technology is used. Analyses will be carried out with the aid of chromatography. The conversion of industrial wastewater to VFA is done by means of acidogenesis in a sequencing batch reactor (SBR). For exploring the trigger effect of a hydrophobic carbon source on MEL production by P. aphidis, an RT-qPCR assay is first developed. This project forms a bridge between different areas of research within the research group Bio-Chemical Green Engineering & Materials (BioGEM) at the Faculty of Applied Engineering (FTI). These are water technology, industrial biotechnology and green and renewable materials.

Researcher(s)

• Promoter: Dries Jan
• Co-promoter: D'aes Jolien
• Fellow: Akkermans Veerle

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The project is a cooperation between Pantarein Water and the the research group BioGEM. The main objective is the development of an innovative water reuse concept that integrates the advantages of aerobic granular sludge (fast settling dense and highly active sludge granules) and membrane processes (water reuse potential). The research hypothesis is that the application of aerobic granular sludge in membrane bioreactors significantly lowers the rate of membrane fouling.

Researcher(s)

• Promoter: Dries Jan
• Fellow: Stes Hannah

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

Aerobic granular sludge represents a revolutionary new biological treatment system based on the formation of very dense microbial aggregates. An interesting feature of the granules is the high content of alginates, which are extracellular biopolymers with valuable applications. The aims of the current project are (1) the maximization of the alginate yield, and (2) the optimization of the polymer extraction procedure.

Researcher(s)

• Promoter: Dries Jan
• Co-promoter: D'aes Jolien

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project type(s)

• Research Project

Abstract

The project involves the pilot-scale demonstration of innovative nitrogen removal from slaughterhouse wastewater by nitritation/denitritation in a conventional activated sludge plant. A shortcut in de nitrogen cycle via nitrite (in stead of nitrate) reduces aeration requirements by 25% (for nitrification) and COD demand by 40% (for denitrification).

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project website

Project type(s)

• Research Project

Abstract

The aim of this research project is to achieve and hold a stable and profound (>80% via nitrite) nitritation in granular sludge. Trough combine this two technologies, significant advantages in terms of energy – efficiency (saving 25% O2, 40% COD and settle times) and land use (saving till 75%) can be obtained. The harmonization of the factors that benefits on one hand the granulation and on the other the nitritation will be the main challenge of this research.

Researcher(s)

• Promoter: Dries Jan
• Co-promoter: Blust Ronny
• Co-promoter: Geuens Luc
• Fellow: Dobbeleers Thomas

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project website

Project type(s)

• Research Project

Abstract

The global aim of the project is to determine the optimal control strategy to achieve a stable and significant degree of partial nitrification/denitrification in conventional activated sludge systems. The application of this technology results in some major advantages in terms of energy and process efficiency: 25% less oxygen needed during nitrificatio, 40% less COD required for denitrification...

Researcher(s)

• Promoter: Dries Jan

Research team(s)

• Biochemical Wastewater Valorization & Engineering (BioWaVE)

Project website

Project type(s)

• Research Project

Abstract

There is a growing concern about the presence of endocrine disrupting compounds (EDCs) in surface waters, caused by discharges from wastewater treatment plants. The current project aims at the optimisation of both conventional and advanced treatment technologies to remove EDCs from industrial wastewater. Treatment efficiencies will be determined by effect directed analysis using bioassays that actually measure the endocrine disrupting effects in the samples.

Researcher(s)

• Promoter: Blust Ronny
• Co-promoter: Dries Jan
• Co-promoter: Geuens Luc

Research team(s)

Project type(s)

• Research Project

Abstract

The PACT process is an advanced waste water treatment technology where powdered activated carbon is added to the activated sludge (1) to protect the treatment process, and (2) to obtain an effluent with an improved chemical quality. In view of the more stringent industrial discharge limits that also include ecological criteria, the current project aims at optimizing the PACT process to obtain effluents with a good biological quality.

Researcher(s)

• Promoter: Blust Ronny
• Co-principal investigator: Dries Jan
• Co-principal investigator: Geuens Luc

Research team(s)

Project website

Project type(s)

• Research Project

Abstract

In this project a process control strategy for batch and continuous flow active sludge wastewater treatment systems will be developed. The control strategy, based on fuzzy logic and characterisation by a newly developed respirometry based device, will allow significant economical as well as ecological advantages. The project also contains an important valorisation part, which will result in an applicable process control strategy.

Researcher(s)

• Promoter: Blust Ronny
• Co-principal investigator: Dries Jan

Research team(s)

Project type(s)

• Research Project

Abstract

The water frame work directive, the new European legislation for water management requires the water quality to fulfill some strict chemical and biological criteria. The duration of classical bioassays is too long and incompatible with the dynamics of a water treatment plant. There is therefore a high need for fast and sensitive bioassays. In this project some fast assays will be validated for the usefulness in waste water treatment applications.

Researcher(s)

• Promoter: Blust Ronny
• Co-principal investigator: Dries Jan
• Co-principal investigator: Geuens Luc
• Co-promoter: Robbens Johan

Research team(s)

Project type(s)

• Research Project