Application of agro-industrial side-streams for the investigation of triggering effects on very-long-chain fatty acid production by Rhodococcus strains and process optimization.
AbstractVery-long-chain fatty acids (VLCFAs) are fatty acids with a chain length of more than 20 carbons. They represent a valuable class of chemicals which can be used in several industries. Nowadays, these are produced from vegetable oils and petrochemical feedstock. In order to make the VLCFA production more sustainable, microbial VLCFA synthesis by Rhodococcus species seems to be a perfect tool. Unfortunately, current knowledge on VLCFA production with Rhodococcus is limited. Therefore, this project proposes to fill this gap. For investigation of the triggering effects to achieve high VLCFA production different agro-industrial substrates will be used as raw material. The triggering effect of the compounds on gene expression of the VLCFA pathway will be evaluated by RT-qPCR. The desired triggers should increase the VLCFA titer. Different Rhodococcus strains will be examined and evaluated for VLCFA synthesis. VLCFA production will be further optimized by screening of several medium compositions, optimization of environmental factors and, finally, the feeding pattern. Establishment of high VLCFA productivities can lead to a new production process of valuable building blocks for chemical industry.
Post-fermentative production of a 2nd generation mannosylerythritol lipid biosurfactants for specialty applications.
AbstractThis project represents a research agreement between the UA and on the onther hand IWT. UA provides IWT research results mentioned in the title of the project under the conditions as stipulated in this contract.
Analysis of mannosyl erythritol lipid biosurfactants and screening of specific antimicrobial activities (BACTIMEL).
AbstractComplex mixtures of mannosyl erythritol lipid (MEL) biosurfactants produced via fermentation will be purified and analytically characterized by liquid chromatography and mass spectrometry. The pure compounds will be screened for their activity against bacteria and fungi.
Time of Flight Mass Spectrometer coupled to Liquid Chromatography system (LC-TOF/MS).
AbstractThis project represents a formal research agreement between UA and on the other hand the Flemish Public Service. UA provides the Flemish Public Service research results mentioned in the title of the project under the conditions as stipulated in this contract.
- Promotor: Neels Hugo
- Co-promotor: Blust Ronny
- Co-promotor: Covaci Adrian
- Co-promotor: Jorens Philippe
- Co-promotor: Wijnants Marc
Ion mobility high resolution mass spectrometer: a strong tool for fragile structures.
AbstractThis project has two general objectives: (1) Increase the research potential of the UA by introduction of new state of the art techniques for the analysis of fragile molecular structures by using the novel ion mobility capabilities that have recently been integrated with high-mass high-resolution Q-TOF mass spetrometry ("Synapt", waters). (2) Maintain the current capacity to obtain Q-TOF data by replacing an existing system.
- Promotor: Sobott Frank
- Co-promotor: Guisez Yves
- Co-promotor: Lemière Filip
- Co-promotor: Maes Bert
- Co-promotor: Moens Luc
- Co-promotor: Tavernier Serge
- Co-promotor: Van Ostade Xaveer
- Co-promotor: Van Schil Paul
- Co-promotor: Wijnants Marc
- Co-promotor: Witters Erwin