Not so complicated...

This picture shows some cardiovascular variables obtained through a method to determine blood flow called pulsed Doppler flowmetry and recorded using a computer-based system (IdeeQ). This technique allows us to make measurements of blood flow in three different vascular beds, while at the same time measuring heart rate and blood pressure, in conscious, freely moving rats. In order to evaluate these changes in the dynamics of the blood flow (haemodynamic), Doppler flow probes need to be surgically placed on the studied vessels. Several parameters will then be recorded using a computer-based system ^1,2. Because the experiments are performed in a live rat, this method also permits us to measure haemodynamic changes in different parts of the vascular system, with reflex systems intact; these are often affected by anesthetics.

During my PhD project I will use the pulsed Doppler flowmetry to investigate changes in mesenteric, renal and hindquarters vascular conductance, as well as heart rate and mean arterial pressure, in rats treated with anticancer drugs targeting vascular endothelial growth factor (VEGF) signalling. Moreover, this approach will also provide essential information to clearly define the mechanism of action underlying cardiovascular impairment due to such novel targeted therapies used in cancer treatment. This project will give us more detailed information about the safety pharmacology issues associated with these treatments ³. 

References:

1. Haywood, J.R., Shaffer, R.A., Fastenow, C., Fink, G.D. & Brody, M.J. Regional blood flow measurement with pulsed Doppler flowmeter in conscious rat. American Journal of Physiology-Heart and Circulatory Physiology 241, H273-H278 (1981). 
2. Gardiner, S.M., Compton, A.M., Bennett, T. & Hartley, C.J. Can pulsed Doppler technique measure changes in aortic blood flow in conscious rats? American Journal of Physiology-Heart and Circulatory Physiology 259, H448-H456 (1990). 
3. Poster SPS meeting 2019, Barcelona