13/12/2022 - Charles (ESR #9)
The image on the flyer shows the distribution of a specific lipid (highlighted in red) in immune cells (macrophages) using Mass Spectrometry Imaging (MSI). The left image shows the MSI image, whereas the right part shows an image acquired by light microscopy. Since its discovery more than 30 years ago, MSI scientists have been trying to push the boundaries of this technology. One major challenge of MSI has been the limited spatial resolution, i.e. the minimum length scale of an acquired image. However, in recent years, more powerful MSI instruments have been developed that allow users to image single cells and reach even subcellular resolution.
Together with my colleague Brigitta Szabó (ESR #1), PhD fellow at Ncardia, we will optimize a protocol to evaluate Doxorubicin distribution at a subcellular level in order to better understand the molecular mechanisms of this drug.